1,2-Dioleoyl-3-(pyren-1-yl) decanoyl-rac-glycerol

Product ID: M0258



Unit SizePriceQuantity 
1mg
$148.15
  • Buy 5 for $118.51 each and save 21%

Availability: In stock


Description

This fluorescent triglyceride analog is an excellent substrate for lipoprotein lipase and hepatic triacylglycerol lipase detection.

The fluorescent triglyceride analog is useful for measuring cholesteryl ester transfer and protein-mediated triacylglycerol transport between plasma lipoproteins. It is also a popular substrate for measuring lipoprotein lipase and hepatic triacylglycerol lipases. The released pyrene-decanoic acid shows a change in excimer formation upon enzyme activity. See also our Product M0612 for more information.

Technical Data
SKU M0258
CAS Number 102807-51-2
Unit Size 1mg
Absorption 342nm
Emission Wavelength 470
Extinction 40
Detection Method Fluorescence
Molecular Formula C₆₅H₉₈O₆
Molecular Weight 975.46
Soluble In CHCl3, DMSO, MeOH
Storage Conditions -20C, Desiccated
Notes Em=377nm is the emission of the pyrenedecanoic acid monomer released upon lipase enzyme activity.

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References and Citations

Citations:

  • Bhatla SC, Vandana S, Kaushik V. (2009) "Recent developments in the localization of oil body-associated signaling molecules during lipolysis in oilseeds." Plant Signal Behav. 4(3):176-82.
  • Millar JS, Duffy D, Gadi R, Bloedon LT, Dunbar RL, Wolfe ML, Movva R, Shah A, Fuki IV, McCoy M, Harris CJ, Wang MD, Howey DC, Rader DJ. (2008) "Potent and selective PPAR-alpha agonist LY518674 upregulates both ApoA-I production and catabolism in human subjects with the metabolic syndrome." Arterioscler Thromb Vasc Biol. 29(1):140-6
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References:

  • "Cholesteryl ester transfer protein reaction between plasma lipoproteins." Main LA, Okumura-Noji K, Ohnishi T, Yokoyama S. J Biochem (Tokyo) 124: 237-243 (1998).
  • "Spectrofluorometric determination of lipase activity." Liodakis A, Drew J, Chan RY, Sawyer WH. Biochem. Int.23(5): 825-34 (1991).
  • "Topo-dynamic characteristics of human plasma VLDL apolipoproteins and efficiency of triacylglycerol hydrolysis by lipoprotein lipase." Dergunov AD, Shuvaev VV, Perova NV. Biochim. Biophys. Acta 1005(1): 79-86 (1989).
  • "Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase." Dousset N, Negre A, Salvayre R, Rogalle P, Dang QQ, Douste-Blazy L. Lipids 23: 605-608 (1988).
  • "Transversal mobility in bilayer membrane vesicles: use of pyrene lecithin as optical probe." Galla HJ, Theilen U, Hartmann W. Chem. Phys. Lipids 23: 239-251 (1979).
  • "Salvayre R., Negre, A., Radom, J., Douste-Blazy, L., (1986) "Fluorometric Assay for Pancreatic Lipase" CLIN. CHEM. 32(8): 1532-1536.
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