10-Octadecylacridine orange bromide

Product ID: M1076

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Useful fluorescent mitochondrial membrane probe used to analyze mitochondria by flow cytometry and measure changes in mitochondrial mass during apoptosis.

Octadecylacridine orange is used to analyze mitochondria by flow cytometry and measure changes in mitochondrial mass during apoptosis. The phospholipid cardiolipin (CL), is found almost exclusively in the inner membrane of mitochondria and loss of CL is considered as an important indication of cell apoptosis. 10-N-octadecyl acridine orange (C18AO) has been used as a fluorescent probe for the visualization of CL in mitochondrial cell membranes and in solution, with excitation and emission wavelengths at about 500 and 525 nm, respectively.

Technical Data
SKU M1076
CAS Number 75168-16-0
Unit Size 20mg
Alternative Names Acridine orange-10-octadecyl bromide
Absorption 495nm
Emission Wavelength 520
Detection Method Fluorescence
Molecular Formula C₃₅H₅₆BrN₃
Molecular Weight 598.74
Soluble In DMSO, MeOH, CH₂Cl₂
Storage Conditions 2-8C

References and Citations


  • Ratinaud MH, Leprat P, Julien R. (1988) "In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes."Cytometry 9: 206.
  • Maftah A, Petit JM, Julien R. (1990) "Specific interaction of the new fluorescent dye 10 N-nonyl acridine orange with inner mitochondrial membrane. A lipid-mediated inhibition of oxidative phosphorylation."FEBS Lett. 260: 236.
  • Kessel, D., (1991) "Characterization of multidrug resistance by fluorescent dyes." Cancer Res. 51: 4665.
  • Cossarizza, A., (1994) "Mitochondrial modifications during rat thymocyte apoptosis: a study at the single cell level." Exp. Cell Res. 214: 323.
  • "Investigation of potential-sensitive fluorescent dyes for application in nitrate sensitive polymer membranes." Mohr GJ, Lehmann F, Ostereich R, Murkovic I, Wolfbeis OS. J Anal Chem 357(3): 284-291 (1997).
  • "Fluorescent potential-sensitive dyes for use in solid state sensors for potassium ion." Murkovic I, Lobnik A, Mohr GJ, Wolfbeis OS. Anal Chim Acta 334(1-2): 125-132 (1996).
  • "Effects of an aliphatic tail on the interactions of an ionic dye and a polyelectrolyte. The system of N-alkylated acridine orange derivatives and poly(styrenesulfonic acid)." Yamagishi A, Watanabe F. J Phys Chem 85(14): 2129-2134 (1981).
  • "Selective activation of reactant molecules by reversed micelles." Yamagishi A, Masui T, Watanabe F. J Phys Chem 85(3): 281-285 (1981).
  • "Distinguishing of molecular size by micelles." Yamagishi, A. Koen Yoshishu - Bunshi Kozo Sogo Toronkai: 50-51 (1979).
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