MarkerGene™ 1X Flow Holding and Sorting Buffer for Non-Adherent Cells

Product ID: M1926



Unit SizePriceQuantity 
60mL
$22.94
  • Buy 5 for $18.35 each and save 21%

Availability: In stock


Description

Shipped in amber Nalgene bottles to prevent ambient light-induced toxicity, the sterile filtered FLOW HOLDING AND SORTING BUFFERS will:

i) Improve cell viability
ii) Enhance and maintain fluorescent signals
iii) Reduce background

For all flow applications that require long ambient conditions, such as sorting or long analytical runs in pursuit of rare events.

Technical Data
SKU M1926
Unit Size 60mL
Storage Conditions Refrigerate
Notes Use M1925 and M1926 for non-adherent cells. Use M1927 and M1928 for adherent cells as they contain EDTA but no Calcium or Magnesium.

References and Citations

References:

  • Spierenburg GT, Oerlemans FT, van Laarhoven JP, de Bruyn CH. (1984) “Phototoxicity of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered culture media for human leukemic cell lines.” Cancer Res. 44(5):2253-4.
  • 5. Zigler JS Jr, Lepe-Zuniga JL, Vistica B, Gery I. (1985) “Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium.” In Vitro Cell Dev Biol. 21(5):282-7.
  • 6. Lepe-Zuniga JL, Zigler JS Jr, Gery I. (1987) “Toxicity of light-exposed Hepes media.” J Immunol Methods. 103(1):145.
  • 8. Lelong IH, Rebel G. (1998) “pH drift of "physiological buffers" and culture media used for cell incubation during in vitro studies.” J Pharmacol Toxicol Methods 39(4):203-10.
  • 9. Wang. RJ, Nixon, BT. (1978) Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with "daylight" fluorescent light. In Vitro, 14: 715-722.
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