2', 7'-Dichlorofluorescin diacetate

Product ID: M0807

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Reduced derivative of 2',7'-dichlorofluorescein is a cell permeable reagent for use in detection of peroxidase activity (oxidative metabolism, intracellular regulation of reactive oxygen species or oxidative burst) inside living cells.

This well known ROS probe can be used to detect reactive oxygen species in live cells. Upon enzyme activity, the highly fluorescent dye 2',7'-dichlorofluorescein Product M1278 is produced, with EX: 495nm and EM: 529 nm. See also Product M0545.

Technical Data
SKU M0807
CAS Number 4091-99-0
Unit Size 25mg
Detection Method Fluorescence
Molecular Formula C₂₄H₁₆O₇Cl₂
Molecular Weight 487.3
Soluble In DMSO, absolute EtOH
Storage Conditions -20C, Desiccated
Notes Prepare solution in ethanol or DMSO and then add to media.

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References and Citations


  • Hsu WH, Chang CC, Huang KW, Chen YC, Hsu SL, Wu LC, Tsou AP, Lai JM, Huang CY. (2015) "Evaluation of the medicinal herb Graptopetalum paraguayense as a treatment for liver cancer." PLoS One. 10(4):e0121298.
  • Yu J, Xu Y, Khaoustov V, Yoffe B. (2011) "Identification of components of grape powder with anti-apoptotic effects." Toxicol Ind Health. 27(1):19-28.
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  • Kalivendi SV, Kotamraju S, Cunningham S, Shang T, Hillard CJ, Kalyanaraman B. (2003) "1-Methyl-4-phenylpyridinium (MPP+)-induced apoptosis and mitochondrial oxidant generation: role of transferrin-receptor-dependent iron and hydrogen peroxide." Biochem J 371:151-64.
  • Tampo Y, Kotamraju S, Chitambar CR, Kalivendi SV, Keszler A, Joseph J, Kalyanaraman B. (2003) "Oxidative stress-induced iron signaling is responsible for peroxide-dependent oxidation of dichlorodihydrofluorescein in endothelial cells: role of transferrin receptor-dependent iron uptake in apoptosis." Circ Res 92:56-63.
  • He YY, Hader D.(2002) "Reactive oxygen species and UV-B: effect on cyanobacteria." Photochem Photobiol Sci 1: 729-36.
  • Burkitt MJ, Wardman P. (2001) "Cytochrome C is a potent catalyst of dichlorofluorescin oxidation: implications for the role of reactive oxygen species in apoptosis." Biochem Biophys Res Commun 282: 329-333.
  • Hail N Jr, Lotan R. (2000) "Mitochondrial permeability transition is a central coordinating event in N-(4-hydroxyphenyl)retinamide-induced apoptosis." Cancer Epidemiol Biomarkers Prev 9: 1293-1301.
  • Salata RA, Sullivan JA, Mandell GL.(1983) "Visualization of hydrogen peroxide in living polymorphonuclear neutrophils utilizing leucodiacetyl 2',7'-dichlorofluorescin: photomicrographic and microphotometric studies." Trans Assoc Am Physicians 96: 375-383.
  • Keston AS, Brandt R.(1965) "The fluorometric analysis of ultramicro quantities of hydrogen peroxide." Anal Biochem 11: 1-5.
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