3-Carboxyumbelliferyl β-D-galactoside, BSA conjugate

Product ID: M0611

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Useful for detection of extracellular βgalactosidase activity in enzyme-linked antibody systems or for endocytosis experiments, measurement of lacY permease activity, or microinjection analyses.

A conjugate of BSA (bovine serum albumin, MW = 66.4K) and the beta-galactosidase substrate carboxyumbelliferyl beta-D-galactopyranoside (Product M0257) produces a protein that can be used to measure extracellular β-galactosidase activity, measure lacY permease activity, for microinjection analyses, or measure cell disruption or lysis. The resulting coumarin-BSA conjugate has fluorescence excitation and emission spectra similar to 3-carboxyumbelliferone (Ex = 386nm; EM = 445nm, e=32K) (blue fluorescence). Note: The fluorescence spectrum for 3-carboxyumbelliferone is pH-dependent. Below the pKa (7.8), Abs shifts to shorter wavelengths (325-340 nm) and fluorescence intensity decreases. These shifts may be used to measure compartmentalization of intracellular activity. See the references below for applications of this substrate for the detection of galactosidase activity.

Technical Data
SKU M0611
Unit Size 2mg
Detection Method Fluorescence
Molecular Weight 66800
Storage Conditions -20C, Desiccated

References and Citations


  • Armenta, R., Tarnowski, T., Gibbons, I., Ullman, E.F.,"Improved sensitivity in homogeneous enzyme immunoassays using a fluorogenic macromolecular substrate: an assay for serum ferritin." (1985) Anal. Biochem. 146:211-219
  • Holst, B.D., Vanderklish, P.W., Krushel, L.A., Zhou, W., Langdon, R.B., McWhirter, J.R., Edelman, G.M., Crossin, K.L.,"Allosteric modulation of AMPA-type glutamate receptors increases activity of the promoter for the neural cell adhesion molecule, N-CAM." (1998) Proc. Natl. Acad. Sci. USA 95:2597-2602.
  • Burd J.F.,"The homogeneous substrate-labeled fluorescent immunoassay." (1981) Methods Enzymol. 74(C):79-87.
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