4-Hydrazino-7-nitrobenzofurazane hydrazine adduct

Product ID: M1025

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Fluorescent derivatization reagent useful for aliphatic aldehyde detection including labeling of reducing carbohydrates.

This NBD derivative has found use in aliphatic aldehyde detection including labeling of reducing carbohydrates in both TLC and HPLC analysis methods.

Technical Data
SKU M1025
CAS Number 131467-87-3
Unit Size 25mg
Alternative Names NBD-hydrazine
Emission Wavelength 522
Detection Method Fluorescence
Molecular Formula C₆H₅N₅O₃ · NH₂NH₂
Molecular Weight 227.18
Storage Conditions 2-8C, Desiccated
Notes Ex= 445nm. EX/EM after derivatization with formaldehyde

References and Citations


  • "Assay for synthesis activity of hydrolases in nonaqueous media using enzymic transesterification reaction releasing a carbonyl compd." Bornscheuer UT, Kanarzycka M, Hills G. Eur. Pat. Appl. EP 1354960 (2003).
  • "Solid reagent for sampling and derivatization of carbonyl compounds." Appelblad P, Irgum K, Ponten E. PCT Int. Appl. WO 2002094769 (2002).
  • "Semi-empirical PM3 calculation reveals the relationship between the fluorescence characteristics of 4,7-disubstituted benzofurazan compounds, the LUMO energy and the dipole moment directed from the 4- to the 7-position." Uchiyama S, Santa T, Imai K. J Chem. Soc., Perkin Transactions 2: Physical Organic Chemistry 3: 569-576 (1999).
  • "Effects of the substituent groups at the 4- and 7-positions on the fluorescence characteristics of benzofurazan compounds." Uchiyama S, Santa T, Fukushima T, Homma H, Imai K. J Chem. Soc., Perkin Transactions 2: Physical Organic Chemistry 10: 2165-2174 (1998).
  • "High-performance liquid chromatography of aliphatic aldehydes by means of post-column extraction with fluorometric detection." Koizumi H, Suzuki Y. J of Chromatography 457: 299-307 (1988).
  • "Fluorogenic labeling of carbonyl compounds with 7-hydrazino-4-nitro-2,1,3-benzoxadiazole (NBD-H)." Guebitz G, Wintersteiger R, Frei RW. J of Liquid Chromatography 7(4): 839-54 (1984).
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