Widely used fluorophore that has the unusual environmentally sensitive property of increasing fluorescence in low pH environments.
This common fluorescent reagent often used in carbohydrate analysis, is also useful in fluorescence visualization of lysosomes in live cell lines in culture. Comparison of staining patterns with the common LysoTracker™ or LysoSensor™ dyes indicated comparable staining, albeit using a higher concentration of NBD-piperazine (1 μM versus 100 nM for the MP dyes), but at a lower cost per assay.
Ishiguro K, Ando T, Goto H, (2008) “Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells.”BioTechniques 45(4): 465-468.
Ghosh PB, Whitehouse MW, (1968) “7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines.” Biochem. J. 108:155-156.
Toyo'oka TM, Ishibashi Y, Takeda K, Nakashima S, Akiyama S, Uzu S, Imai K, (1991) “Precolumn fluorescence tagging reagent for carboxylic acids in high-performance liquid chromatography: 4-substituted-7-aminoalkylamino-2,1,3-benzoxadiazoles.” J. Chromatogr. 588:61-71.
Toyo'oka TM, (2002) “Fluorescent tagging of physiologically important carboxylic acids, including fatty acids, for their detection in liquid chromatography.” Anal. Chim. Acta 465(1-2): 111-130.
Santa T, Matsumura D, Huang C, Kitada C, Imai K, (2002) “Design and synthesis of a hydrophilic fluorescent derivatization reagent for carboxylic acids, 4-N-(4-N-aminoethyl)piperazino-7-nitro-2,1,3-benzoxadiazole (NBD-PZ-NH2), and its application to capillary electrophoresis with laser-induced fluorescence detection.” Biomed. Chromatogr. 16:523-528.
Fehrenbacher N, Jaattela M, (2005) “Lysosomes as targets for cancer therapy.” Cancer Res. 65:2993-2995.
Kroemer G, Jaattela M, (2005) “Lysosomes and autophagy in cell death control.” Nat. Rev. Cancer 5:886-897.
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