Brominated analog of carboxyfluorescein that is a useful photosensitizer and singlet oxygen generator with SE ester for conjugations.
Carboxyeosin has a singlet oxygen yield that is approximately 19 times greater than that of fluorescein. Using this property, CE has been found to be useful for photoconverting diaminobenzidine (DAB) as a method for improving resolution for immunoprecipitation or in situ hybridization techniques in light and electron microscopy. Upon excitation, carboxyeosin labeled probes can be used to generate singlet oxygen, which in turn will oxidize DAB into an opaque precipitate within cells. The resulting DAB reaction provides a nondiffusible staining method for monitoring hybridization or antibody localization in cells and tissues. Carboxyeosin has also been found to be a sensitive, non-covalently bound fluorescent probe for monitoring conformational changes in detergent-solubilized Na,K-ATPase as well as plasma membrane Ca+2 ATPase activity. Finally, carboxyeosin is also a well-known, specific PMCA (plasma membrane calcium pump) inhibitor.
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