5(6)-Carboxyfluorescein, NHS ester

Product ID: M0984

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Useful fluorescent amine-reactive probe for synthesis of bioconjugates, yielding hydrolysis resistant derivatives which show excellent performance and stability at physiological pH values.

FL-NHS is a widely used reactive dye for labeling of bioconjugates on nucleophilic sites (amino-terminus, lysine residues, Amino C6 Linker containing oligonucleotides) yielding hydrolysis resistant derivatives that exhibit excellent performance and stability at physiological pH values.

Technical Data
SKU M0984
CAS Number 117548-22-8
Unit Size 25mg
Alternative Names 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester
Absorption 495nm
Emission Wavelength 520
Extinction 75
Detection Method Fluorescence
Molecular Formula C₂₅H₁₅NO₉
Molecular Weight 473.4
Soluble In DMF, DMSO
Storage Conditions -20C, Desiccated, Protect From Light

References and Citations


  • Kim J, Seidler P, Fill C, Wan LS (2008) "Investigations of the effect of curing conditions on the structure and stability of amino-functionalized organic films on silicon substrates by Fourier transform infrared spectroscopy, ellipsometry, and fluorescence microscopy" Surface Science 602,(21), 3323–3330
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  • "Extending the applicability of carboxyfluorescein in solid-phase synthesis." Fischer R, Mader O, Jung G, Brock R. Bioconjug. Chem. 14: 653-60 (2003).
  • "Protein labeling with fluorescent probes." Holmes KL, Lantz LM. Methods Cell. Biol. 63: 185-204 (2001).
  • "A mild method for fluorescein labeling of base-sensitive oligonucleotides on solid support." Bologna JC, Imbach JL, Morvan F. Tetrahedron Lett. 41: 7317 (2000).
  • "Simultaneous detection of TaqMan probes containing Fam and Tamra reporter fluorophores." Nasarabadi S, Milanovich F, Richards J, Belgrader P. Biotechniques 27: 1116-1118 (1999).
  • "Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes." Li Y, Glazer AN. Bioconjug. Chem. 10: 241-245 (1999).
  • "Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes." Fuchs BM, Wallner G, Beisker W, Schwippl I, Ludwig W, Amann R. Appl. Environ. Microbiol. 64: 4973-4982 1998).
  • "Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis." Banks PR, Paquette DM. Bioconj. Chem. b>6: 447 (1995).
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