Product ID: M1248

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This pH-dependent fluorophore with red fluorescence (EX 598/ EM 668nm at pH 9) has a high pKa (7.6) value. The fluorescence shifts to shorter wavelengths (EX 512/ EM 567nm) at neutral or acidic pH environments.

Carboxynaphthofluorescein has been exploited in both biological and industrial applications, including the construction of fiber-optic pH sensors.

Technical Data
SKU M1248
CAS Number 128724-35-6
Unit Size 25mg
Absorption 598nm
Emission Wavelength 668
Detection Method Fluorescence
Molecular Formula C₂₉H₁₆O₇
Molecular Weight 476.43
Soluble In MeOH, DMSO
Storage Conditions Room Temperature

References and Citations


  • Rogalski, Melissa M., "Development of Position-Dependent Luminescent Sensors: Spectral Rulers and Chemical Sensing Through Tissue" (2016). All Dissertations. Paper 1706
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  • de Lamirande E, Gagnon C, (1998) "Paradoxical effect of reagents for sulfhydryl and disulfide groups on human sperm capacitation and superoxide production." Free Radical Biol. Med. 25: 803-817.
  • Song A, Parus S, Kopelman R, (1997) "High-performance fiber-optic pH microsensors for practical physiological measurements using a dual-emission sensitive dye." Anal. Chem. 69: 863-867.
  • Fabian WMF, Schuppler S, Wolfbeis OS, " (1996) in "Effects of Annulation on Absorption and Fluorescence Characteristics of Fluorescein Derivatives: a Computational Study." p. 853.
  • Wolfbeis OS, Rodriguez NV, Werner T,(1992) "LED-Compatible Fluorosensor for Measurement of Near-Neutral pH Values." Mikrochim. Acta 108: 133.
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