5-Carboxy-X-Rhodamine, NHS Ester

Product ID: M1471



Unit SizePriceQuantity 
5mg
$336.71
  • Buy 5 for $269.37 each and save 20%

Availability: In stock


Description

This dye is commonly used in dye-terminator sequencing methods by attachment to dideoxynucleotide triphosphates (ddNTP's). It can also be used in other DNA or RNA labeling methods.

It can also be used in other DNA or RNA labeling methods.

Technical Data
SKU M1471
CAS Number 209734-74-7
Unit Size 5mg
Alternative Names 5-ROX, NHS
Absorption 578nm
Emission Wavelength 599
Detection Method Fluorescence
Molecular Formula C₃₇H₃₃N₃O₇
Molecular Weight 631.68
Storage Conditions -20C, Desiccated, Protect From Light

References and Citations

References:

  • Mackay IM (2004) "Real-time PCR in the microbiology laboratory." Clin. Microbiol. Infect. 10(3): 190-212.
  • Ju J, Ruan C, Fuller CW, Glazer AN, Mathies RA (1995) "Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis." Proc. Natl. Acad. Sci. USA (92):10 4347-4351.
  • Ansorge W, Posenthal A, Sproat B, Stegemann J, Voss H, (1988) "Non-radioactive automated sequencing of oligonucleotides by chemical degradation." Nucleic Acids Res. 16: 2203-2206.
  • Prober JM, Trahnor SH, Dann RJ, Hobbs FW, Robertson CW, Zagursky RJ, Cocuzza AJ, Jensen MA, Baumeister K (1987) "A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides." Science 238: 336-341.
  • Smith LM, Fung S, Hunkapiller MW, Hunkapiller TJ, Hood LE (1986) "The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis." Nucleic Acids Res. 13: 2399-2412.
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