Product ID: M1059

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Blue fluorescent reagent useful for preparing aminopeptidase substrates.

7-Amino-4-methylcoumarin (AMC) is a well-known laboratory reagent employed in the preparation of fluorogenic AMC-based substrates for the detection of proteolytic enzyme activity. AMC has been utilized as a fluorescent probe to analyze glycoproteins' monosaccharides and N-linked oligosaccharides via chromatography and has also been used as a laser dye. Recent research shows that AMC was extracted from an endophytic Xylaria fungal species and exhibited strong antibacterial and antifungal activities in vitro against Aspergillus, Candida, Escherichia, Staphylococcus, Salmonella and Vibrio species, among others. This product is also useful as a reference standard in enzymatic assays. See our Product M1060, L-Leucine 7-amido-4-methyl coumarin Hydrochloride, for more details.

Technical Data
SKU M1059
CAS Number 26093-31-2
Unit Size 100mg
Alternative Names coumarin 120, AMCA, AMC
Absorption 380nm
Emission Wavelength 440
Detection Method Fluorescence
Molecular Formula C₁₀H₉NO₂
Molecular Weight 175.18
Soluble In DMSO, MeOH
Storage Conditions Room Temperature, Desiccated

References and Citations


  • "Interactions of phage P22 tails with their cellular receptor, Salmonella O-antigen polysaccharide." Baxa U, Steinbacher S, Miller S, Weintraub A, Huber R, Seckler R. Biophys J 71: 2040-2048 (1996).
  • "Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay." Irvine GB, Ennis M, Williams CH. Anal Biochem 185: 304-307 (1990).
  • "Studies on immunoassays of peptide factors. II. Fluorescence enzyme immunoassay for human little-gastrin." Moroder L, Bovermann G, Mourier G, Gohring W, Gemeiner M, Wunsch E. Biol Chem Hoppe Seyler 368: 831-838 (1987).
  • "A new fluorescent tag for labeling of saccharides." Prakash C, Vijay IK. Anal Biochem 128: 41-46 (1983).
  • "Synthesis of Fluorescent Probe - Carbohydrate Conjugates." Yalpani M, Hall LD. Can J Chem 59: 2934 (1981).
  • "Substituted Coumarins and Azacoumarins. Synthesis and Fluorescent Properties." Atkins RL, Bliss DE. J Org Chem 43: 1975 (1978).
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