7-(Diethylamino)coumarin-3-carbonyl azide

Product ID: M0853

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Diethylaminocoumarin-3-carbonyl azide is a aldehyde and ketone reactive probe useful for labeling proteins or other carbonyl containing compounds. It also has found use in HPLC labeling of lipids and alkylcycloketones.

Ceramides can be derivatized using 7-(diethylamino)coumarin-3-carbonyl azide with subsequent gradient HPLC separation allowing sensitive optical quantification of individual cellular ceramides. It also has found use in HPLC labeling of lipids and alkylcycloketones.

Technical Data
SKU M0853
CAS Number 157673-16-0
Unit Size 25mg
Absorption 390nm
Emission Wavelength 478
Extinction 57
Detection Method Fluorescence
Molecular Formula C₁₄H₁₄N₄O₃
Molecular Weight 286.29
Soluble In DMSO, MeOH, DMF, CH3CN
Storage Conditions -20C, Desiccated, Protect From Light

References and Citations


  • Mörsel JT, Schmiedl D, (1994) "Determination of 2-alkylcyclobutanone using fluorescent labeling. "Fresenius Z. Anal. Chem. 349: 538.
  • Balestrieri C, Camussi G, Giovane A, Lorio EL, Quangliuolo L, Servillo L, (1996) "Measurement of platelet-activating factor acetylhydrolase activity by quantitative high-performance liquid chromatography determination of coumarin-derivatized 1-O-alkyl-2-sn-lysoglyceryl-3-phosphorylcholine. Analytical Biochemistry 233(2):45-50.
  • Chatterjee SK, Laffray J, Patel P, Rudravajhala R, Yong Q, Kuehne ME, Bane SL, (2002) "Interaction of Tubulin with a New Fluorescent Analogue of Vinblastine." Biochemistry 41(47): 14010-14018.
  • McKeone BJ, Osmundsen K, Brauchi D, Pao Q, Payton-Ross C, Kilinç C, Kummerow FA, Pownall HJ (1997) "Alterations in serum phosphatidylcholine fatty acyl species by eicosapentaenoic and docosahexaenoic ethyl esters in patients with severe hypertriglyceridemia." J. Lipid Res. 38:429-436.
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