7-Methoxycoumarin-3-carboxylic acid

Product ID: M1021



Unit SizePriceQuantity 
100mg
$65.82
  • Buy 5 for $52.66 each and save 20%

Availability: In stock


Description

An amine-reactive blue fluorescent probe that provides a distinguishable contrast when used with longer-wavelength probes in certain multicolor fluorescence applications such as nucleic acid and protein microarrays, in situ hybridization and immunofluorescence.

This carboxylic acid derivative of 7-methoxycoumarin can be activated in an aqueous solution with water-soluble carbodiimide reagents like EDAC for coupling to amines, hydrazines or hydroxylamines on biomolecules of interest. The resulting labled bioconjugates have blue-fluorescence with absorption/emission maxima at 336/402 nm respectively. These probes have a easily distinguishable contrast when used with longer-wavelength probes in certain multicolor fluorescence applications such as nucleic acid and protein microarrays, in situ hybridization and immunofluorescence.

Technical Data
SKU M1021
CAS Number 20300-59-8
Unit Size 100mg
Alternative Names 2H-1-Benzopyran-3-carboxylic acid
Emission Wavelength 402
Detection Method Fluorescence
Molecular Formula C₁₁H₈O₅
Molecular Weight 220.18
Storage Conditions Room Temperature
Notes EX = 330nm

References and Citations

References:

  • Sherman WR, Robins E. "Fluorescence of substituted 7-hydroxycoumarins." Anal Chem 40, 803-805 (1968).
  • Tisljar U, Knight CG, Barrett AJ. "An alternative quenched fluorescence substrate for Pz-peptidase." Anal Biochem 186, 112-115 (1990).
  • Mita H, Yasueda H, Hayakawa T Shida T. "Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection." Anal Biochem 180, 131-135 (1989).
  • Thaker NN, Trivedi KN. "Mesomorphic Heterocyclic Homologous Series: Cholesteryl Esters of 7-n-Alkoxycoumarin-3-carboxylic Acids & Ethyl Esters of 7-(p-n-Alkoxybenzoylozy)coumarin-3-carboxylic Acids." Indian J Chem 20, 560 (1981).
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