Product ID: M1231

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Fluorogenic Cytochrome P450 and microsomal dealkylase substrate for differentiation of several isoforms of these enzymes producing a blue fluorescence emission upon enzyme activity.

This CYP450 substrate produces the blue fluorescent dye 7-hydroxycoumarin upon enzyme activity. This substrate, along with other alkyl resorufins such as the methyl and benzyl ethers (Products M1209 and M1166) has also been extensively used to differentiate the various isozymes of the Cytochrome P450 system. O-Dealkylations of resorufin and coumarin ethers are mediated by microsomal cytochrome P450 mono-oxygenases from animals, plants and microorganisms. 7-hydroxycoumarin M1280 is produced as a result of enzymatic activity.

Technical Data
SKU M1231
CAS Number 531-59-9
Unit Size 50mg
Alternative Names Methyl umbelliferyl ether, Herniarin
Absorption 350nm
Emission Wavelength 389
Detection Method Fluorescence
Molecular Formula C₁₀H₈O₃
Molecular Weight 176.17
Soluble In DMSO, MeOH(sl), DMF, CH3CN
Storage Conditions Room Temperature, Protect From Light
Notes High Purity (99+%)

References and Citations


  • Celik, A., Roberts, G.A., White, J.H., Chapman, S.K., Turner N.J., Flitsch S.L., (2006) "Probing the substrate specificity of the catalytically self-sufficient cytochrome P450 RhF from a Rhodococcus sp." Chem. Commun., 4492-4494.
  • Kim, K.H., Isin, E.M., Yun, C.H., Kim, D.H., Guengerich, F.P. (2006) "Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes. Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation." FEBS Journal 273(10): 2223-2231.
  • Matsubara, T., Yamada, N., Mitomi, T., Yokoyama, S., Fukiishi, Y., Hasegawa, Y., Nishimura, H., (1986) "Cytochrome P-450-dependent monooxygenase activities in prenatal and postnatal human livers: comparison of human liver 7-alkoxycoumarin O-dealkylases with rat liver enzymes. Japanese Journal of Pharmacology 40(3): 389-98.
  • Lindahl, S. R., Scheline, R. R. (1971), "Metabolism of umbelliferone and herniarin in rats and by the rat intestinal microflora." Xenobiotica 1(1): 13-24.
  • Fleck, H. R., Holness, R. F. G., Ward, A. M. (1935) "Some examples of fluorescent acidimetric and adsorption indicators." Analyst 60: 32-3.
  • Warren M., Fry J.R., (1988), "Influence of medium composition on 7-alkoxycoumarin O-dealkylase activities of rat hepatocytes in primary maintenance culture." Xenobiotica 18(8): 973-81.
  • Muthuramu, K., Ramamurthy, V., (1984) "7-Alkoxycoumarins as fluorescence probes for microenvironments." Journal of Photochemistry 26(1): 57-64.
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