8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt

Product ID: M1296

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Highly polar, highly water soluble lipase substrate is a derivative of HPTS (pyranine) that forms tight micelles in aqueous solutions.

Alkyl esters of HPTS (sometimes called Cascade Blue) are well known for their substrate ability to measure esterase activity. Longer chain derivatives function as lipase substrates. Lipase activity can be measured by monitoring the sensitive fluorescence kinetics following the increase in emission of product HPTS (hydroxypyrene trisulfonic acid) as a function of time. The activity of a number of lipases have been measured using this substrate including wheat germ lipase, candida cylindracea lipase as well as carboxylic ester hydrolase and acylase 1. Because of its relatively low pKa value (7.3) under assay conditions, it is partially dissociated to form an anion that shows strong absorption maximizing at 460 nm and fluoresces with very high quantum yield and maximum intensity at 520 nm. Unfortunately, when excited between 440 and 480 nm, the fluorescence intensity is dependent on the pH of the solution, which requires careful pH control. However, when excited at 415 nm (the isosbestic point), no pH dependence of fluorescence intensity at 520 nm is noted.

Technical Data
SKU M1296
CAS Number 115787-84-3
Unit Size 25mg
Alternative Names Octanoyl Pyrenetrisulfonic acid, trisodium salt, Trisodium 8-octanoyloxypyrene-1,3,6-trisulfonate
Absorption 460nm
Emission Wavelength 510
Detection Method Fluorescence
Molecular Formula C₂₄H₂₁Na₃O₁₁S₃
Molecular Weight 650.58
Soluble In H₂O, MeOH, DMSO, DMF
Storage Conditions -20C, Desiccated

References and Citations


  • Koller, E, Wolfbeis, OS, (1988) "Preparation of pyrenesulfonic acid derivatives for photometric determination of enzyme activity." Austrian Patent AT 385755.
  • Kriechbaum M, Wolfbeis OS, Koller E, (1987) "Evaluation of Critical Micelle Concentrations Using New Superpolar Lipid Probes. "Chem. Phys. Lipids 44: 89-94.
  • Baumeister B, Sakai N, Matile, S, (2001) "p-Octiphenyl b -barrels with ion channel and esterase activity." Organic Letters 3(26): 4229-4232.
  • Wolfbeis OS, Koller E, (1983) "Fluorimetric assay of hydrolases at longwave excitation and emission wavelengths with new substrates possessing unique water solubility." Anal Biochem. 129(2):365–370.
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