Molecular Weight: 656.6
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO, DMF, ETOH, H2O
Absorption: (in nm) 300
Molecular Formula: C32H32O15
CAS Number: [17817-20-8]
Alternative Name: Fluorescein di-galactoside; FDG
Unit Price (USD):
5 mg $86.88
Bulk Price/unit (when you buy 5 or more): $69.50
Description:
Ultra-sensitive substrate for lacZ β-galactosidase detection in vivo and in vitro.
Note:
Emission (EX: 488nm / EM: 512nm) of the fluorescein fluorophore released upon beta-Galactosidase enzyme activity.
Molecular Biology grade: <20 ppm background fluorescence QC specification.
Application:
See references below for numerous applications of this widely used substrate.
References:
"Glycogen synthase kinase-3 is an in vivo regulator of hematopoietic stem cell repopulation." Trowbridge JJ, Xenocostas A, Moon RT, Bhatia M. Nature Medicine 12: 89-98 (01 Jan 2006)."Direct influence of S9 liver homogenate on fluorescence signals: impact on practical applications in a bacterial genotoxicity assay." Dreier J, Breitmaier EB, Gocke E, Apfel CM, Page MGP. Mutation Research 513(1-2): 169-182 (2002)."Electrophoretically mediated microanalysis of b-galactosidase on microchips." Burke BJ, Regnier FE. Electrophoresis 22(17): 3744-3751 (2001)."Photoablation of cells expressing beta-galactosidase." Nirenberg S. Methods Mol. Biol. 135: 475-80 (2000). "Enzymatic detection of coliforms and Escherichia coli within 4 hours." Nelis H, Van Poucke S. Water, Air, and Soil Pollution 123(1-4): 43-52 (2000). "Miniaturization of a functional transcription assay in yeast (human progesterone receptor) in the 384- and 1536-well plate format." Berg M, et al. J. Biomol. Screen. 5: 71 (2000)."Fluorescent probes for living cells." Johnson I. Histochem. J. 30: 123 (1998). "A microplate fluorimetric assay for transfection of the beta-galactosidase reporter gene." Rakhmanova VA,MacDonald RC. Anal. Biochem. 257: 234 (1998). "Verapamil inhibition of enzymatic product efflux leads to improved detection of beta-galactosidase activity in lacZ-transfected cells." Poot M, Arttamangkul S. Cytometry 28: 36 (1997). "The light response of retinal ganglion cells is truncated by a displaced amacrine circuit." Nirenberg S, Meister M. Neuron 18(4): 637-50 (1997)."Fluorescence-based enzymatic assay by capillary electrophoresis laser-induced fluorescence detection for the determination of a few beta-galactosidase molecules." Craig D, et al. Anal. Biochem. 226: 147 (1995)."LacZ expression in germline transgenic zebrafish can be detected in living embryos." Lin S, Yang S, Hopkins N. Dev. Biol. 161: 77 (1994). "No intermediate channelling in stepwise hydrolysis of fluorescein di-beta-D-galactoside by beta-galactosidase." Fiedler F, Hinz H. Eur. J. Biochem. 222: 75 (1994)."Beta-galactosidase activity in transfected Ltk-cells is differentially regulated in monolayer and in spheroid cultures." Klunder I, Hulser DF. Exp. Cell Res. 207: 155 (1993). "Beta-galactosidase activity in single differentiating bacterial cells." Russo-Marie F, et al. Proc. Natl. Acad. Sci. USA 90: 8194 (1993)."Rapid identification and quantitation of cells infected by recombinant herpesvirus (pseudorabies virus) using a fluorescence-based beta-galactosidase assay and flow cytometry." Saalmuller A, Mettenleiter TC. J Virol. Methods 44: 99 (1993). "Targeted ablation of diverse cell classes in the nervous system in vivo." Nirenberg S, Cepko C. J Neurosci. 13(8): 3238-51 (1993). "Beta-galactosidase activity in bacteria measured by flow cytometry." Alvarez AM, Ibanez M, Rotger R. BioTechniques 15: 974 (1993). "FACS-Gal: flow cytometric analysis and sorting of cells expressing reporter gene constructs." Roederer M, Fiering S, Herzenberg LA. Methods: Comp. Meth. Enzymol. 2: 248 (1991). "Gene-search viruses and FACS-Gal permit the detection, isolation, and characterization of mammalian cells with in situ fusions between cellular genes and escherichia coli lacZ." Kerr WG, Herzenberg LA. Methods: Comp. Meth. Enzymol. 2: 261 (1991). "Visualizing interleukin 2 gene expression at the single cell level." Emilie D, et al. Eur. J. Immunol. 19: 1619 (1989). "Detection of acid-beta-galactosidase activity in viable human fibroblasts by flow cytometry." Jongkind JF, Verkerk A, Sernetz M. Cytometry 7: 463 (1986). "Beta-D-galactosidase activity in single yeast cells during cell cycle of Saccharomyces lactis." Yashphe J,Halvorson HO. Science 191: 1283 (1976). "Enzyme-linked immunoassay I. Novel method for synthesis of the insulin-beta-D-galactosidase conjugate and its applicability for insulin assay." Kato K, et al. J Biochem. 78: 235 (1975). "Fluorogenic Substrates for beta-D-galactosidases and phosphatases derived from fluorescein (3,6-dihydroxyfluoran) and its monomethyl ether." Rotman B, Zderic JA, Edelstein M. Proc. Natl. Acad. Sci. USA 50: 1 (1963). Guo W, Wu H, (2008) "Detection of LacZ expression by FACS-Gal analysis" Nature Protocols DOI: 10.1038/nprot.2008.163 http://www.natureprotocols.com/2008/08/06/detection_of_lacz_expression_b.php
Keywords:
Fluorescein di-β-D-Galactopyranoside, FDG, Fluorescein, di-β-D-Galactopyranoside, di-Beta-D-Galactopyranoside, di-B-D-Galactopyranoside, Marker Gene
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