Molecular Weight: 460.4
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO
Absorption: (in nm) 300
Extinction (103): 63
Molecular Formula: C25H16O9
CAS Number: [124387-19-5]
Alternative Name: High Purity-Molecular Biology Grade
Unit Price (USD):
100 mg $108.50
Bulk Price/unit (when you buy 5 or more): $86.80
Description:
Popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems
Application:
Especially useful for automated cell viability assays because the cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein Product M0122. This fluorophore is well-retained inside live cells, while the substrate CFDA is membrane permeant. See the references for additional information about CFDA use in bacterial, plant, yeast and mammalian cell culture. Green emission spectral properties (Abs 488 nm / EM 517 nm) after removal of acetate esters, intracellularly.
References:
"A trace amount of the human pro-apoptotic factor bax induces bacterial death accompanied by damage of DNA." Asoh S, Nishimaki K, Nanbu-Wakao R., Ohta S. J Biol. Chem. 273(18): 11384-91 (1998).
"Subcellular compartmentation of different lipophilic fluorescein derivatives in maize root epidermal cells." Brauer D., et al. Protoplasma 192: 70 (1996).
"Role of three Rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast." Singer-Kruger B, Stenmark H, Dusterhoft A, Philippsen P, Yoo JS, Gallwitz D, Zerial M. J Cell. Biol. 125(2): 283-298 (1994).
"Measurement of intracellular fluorescence of human monocytes relative to oxidative metabolism." Robinson JP, Bruner LH, Bassoe CF, Hudson JL, Ward PA, Phan SH. J Leukocyte Biol. 43(4): 304-310 (1988).
Keywords:
Carboxyfluorescein diacetate, Carboxyfluorescein, diacetate, fluorescein, M0011, CFDA, 79955-27-4, 3348-03-6, Marker Gene
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