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M0013 - 5(6)-Carboxyfluorescein diacetate, NHS ester
(5(6)-Carboxyfluorescein N-hydroxysuccinimidyl ester)


Molecular Weight: 557

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO, CH3CN(s)

Emission: (in nm) 517

Molecular Formula: C29H19O11N

CAS Number: [150347-59-4]

Alternative Name: 5(6)-Carboxyfluorescein N-hydroxysuccinimidyl ester

Unit Price (USD): 25 mg $121.54

Bulk Price/unit (when you buy 5 or more): $97.23

Description:
Labeling reagent (amine-reactive) with green emission spectral properties (Abs 488 nm / EM 517 nm) after intracellular removal of acetate esters.

Note:
Emission of fluorophore released upon esterase activity. Labeling reagent for proteins. Amino groups on proteins react with the NHS-ester to form stable amide linked conjugates. Subsequent esterase activity releases the fluorescent carboxyfluorescein (Abs: 475, Em 517).

References:

  • "Calcein: a novel marker for lymphocytes which enter lymph nodes." Weston SA, Parish CR. Cytometry 13(7): 739-749 (1992).
  • "New fluorescent dyes for lymphocyte migration studies : Analysis by flow cytometry and fluorescence microscopy." Weston SA, Parish CR. J. Immunol. Meth. 133: 87-97 (1990).
  • "A novel cadherin cell adhesion molecule: its expression patterns associated with implantation and organogenesis of mouse embryos." Nose A, Takeichi M. J Cell. BioL. 103: 2649-2658 (1986).
  • "Alterations in neural crest migration by a monoclonal antibody that affects cell adhesion." Bronner-Fraser M. J Cell. BioL. 101(2): 610-617 (1985).


  • Keywords:

    150347-59-4, Carboxyfluorescein diacetate, NHS ester, NHS, ester, Carboxyfluorescein, diacetate, M0013, Marker Gene


    ©2012 Marker Gene Technologies, Inc. Published by Marker Gene Technologies, Inc., The University of Oregon Riverfront Research Park, 1850 Millrace Drive, Eugene, Oregon 97403-1992 USA. All rights reserved. For information on the use or copying of the material contained in this document, please contact us at techservice@markergene.com.