Molecular Weight: 557
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO, CH3CN(s)
Emission: (in nm) 517
Molecular Formula: C29H19O11N
CAS Number: [150347-59-4]
Alternative Name: 5(6)-Carboxyfluorescein N-hydroxysuccinimidyl ester
Unit Price (USD):
25 mg $121.54
Bulk Price/unit (when you buy 5 or more): $97.23
Description:
Labeling reagent (amine-reactive) with green emission spectral properties (Abs 488 nm / EM 517 nm) after intracellular removal of acetate esters.
Note:
Emission of fluorophore released upon esterase activity. Labeling reagent for proteins. Amino groups on proteins react with the NHS-ester to form stable amide linked conjugates. Subsequent esterase activity releases the fluorescent carboxyfluorescein (Abs: 475, Em 517).
References:
"Calcein: a novel marker for lymphocytes which enter lymph nodes." Weston SA, Parish CR. Cytometry 13(7): 739-749 (1992)."New fluorescent dyes for lymphocyte migration studies : Analysis by flow cytometry and fluorescence microscopy." Weston SA, Parish CR. J. Immunol. Meth. 133: 87-97 (1990)."A novel cadherin cell adhesion molecule: its expression patterns associated with implantation and organogenesis of mouse embryos." Nose A, Takeichi M. J Cell. BioL. 103: 2649-2658 (1986)."Alterations in neural crest migration by a monoclonal antibody that affects cell adhesion." Bronner-Fraser M. J Cell. BioL. 101(2): 610-617 (1985).
Keywords:
150347-59-4, Carboxyfluorescein diacetate, NHS ester, NHS, ester, Carboxyfluorescein, diacetate, M0013, Marker Gene
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