Molecular Weight: 416.39
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO
Absorption: (in nm) 290
Molecular Formula: C24H16O7
CAS Number: [596-09-8]
Alternative Name: USP Standard
Unit Price (USD):
1 g $46.30
Bulk Price/unit (when you buy 5 or more): $37.04
Description:
Most widely-used green fluorogenic substrate for esterase activity.
Note:
Converted to a fluorescent product with pH-dependent spectra similar to 5(6) carboxyfluorescein Product M0122 , (Abs = 492nm, E = 78K, Em = 517nm) after acetate (esterase) hydrolysis. Useful as a cell viability probe in cell culture. See references below for applications.
References:
"Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements." Deutsch M, Kaufman M, Shapiro H, Zurgil N. Cytometry 39(1): 36-44 (2000)."Probing the physiological state of phytoplankton at the single-cell level." F.J. Jochem. Sci Mar 64: 183 (2000). "Comparative analysis of apoptosis in HL60 detected by annexin-V and fluorescein-diacetate." Bartkowiak D, Hogner S, Baust H, Nothdurft W, Rottinger EM. Cytometry 37(3): 191-196 (1999). "Flow cytometric characteristics of sperm cells isolated from pollen of Zea mays L. Zhang G., et al. Plant Physiol. 99: 54 (1992). "Staining for enzymatic activity after gel electrophoresis." Gersten DM, Gabriel O. Anal. Biochem. 203(2): 181-186 (1992).
Keywords:
Fluorescein diacetate, FDA, Fluorescein, diacetate, M0060, Marker Gene
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