MGT Inc. Login  |  Register  |  Newsletter Subscription  |  Cart 
MGT Inc.
MGT Inc.
MGT Inc.
     

Products


Vectors Vectors
Enzyme Substrates and Kits Enzyme Substrates and Kits
Enzyme Inhibitors Enzyme Inhibitors
MGT Detection Kits MGT Detection Kits
Cell Regulatory Substrates Cell Regulatory Substrates
Special Labeling Reagents Special Labeling Reagents
Lipids Lipids
Carbohydrates/Oligosaccharides Carbohydrates/Oligosaccharides  
Fluorescent Reagents Fluorescent Reagents

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Haiti Relief FundDuring the month of February, Marker Gene will donate 10% from the revenues of all orders placed online at www.markergene.com to the Save the Children’s Support the Haiti Earthquake Children in Emergency Fund. Thanks for your help!

M0060 - Fluorescein diacetate (FDA)
(USP Standard)


Molecular Weight: 416.39

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO

Absorption: (in nm) 290

Molecular Formula: C24H16O7

CAS Number: [596-09-8]

Alternative Name: USP Standard

Unit Price (USD): 1 g $46.30

Bulk Price/unit (when you buy 5 or more): $37.04

Description:
Most widely-used green fluorogenic substrate for esterase activity.

Note:
Converted to a fluorescent product with pH-dependent spectra similar to 5(6) carboxyfluorescein Product M0122 , (Abs = 492nm, E = 78K, Em = 517nm) after acetate (esterase) hydrolysis. Useful as a cell viability probe in cell culture. See references below for applications.

References:

  • "Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements." Deutsch M, Kaufman M, Shapiro H, Zurgil N. Cytometry 39(1): 36-44 (2000).
  • "Probing the physiological state of phytoplankton at the single-cell level." F.J. Jochem. Sci Mar 64: 183 (2000).
  • "Comparative analysis of apoptosis in HL60 detected by annexin-V and fluorescein-diacetate." Bartkowiak D, Hogner S, Baust H, Nothdurft W, Rottinger EM. Cytometry 37(3): 191-196 (1999).
  • "Flow cytometric characteristics of sperm cells isolated from pollen of Zea mays L. Zhang G., et al. Plant Physiol. 99: 54 (1992).
  • "Staining for enzymatic activity after gel electrophoresis." Gersten DM, Gabriel O. Anal. Biochem. 203(2): 181-186 (1992).


  • Keywords:

    Fluorescein diacetate, FDA, Fluorescein, diacetate, M0060, Marker Gene


    ©2010 Marker Gene Technologies, Inc. Published by Marker Gene Technologies, Inc., The University of Oregon Riverfront Research Park, 1850 Millrace Drive, Eugene, Oregon 97403-1992 USA. All rights reserved. For information on the use or copying of the material contained in this document, please contact us at techservice@markergene.com.