Molecular Weight: 494.5
Storage: F, D, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: H2O, MeOH, DMF, DMSO, ETOH
Absorption: (in nm) 452
Emission: (in nm) 518
Molecular Formula: C26H22O10
CAS Number: [102286-67-9]
Unit Price (USD):
5 mg $133.75
Bulk Price/unit (when you buy 5 or more): $107.00
Description:
Convenient β-galactosidase fluorescent probe. Especially useful for enzymatic analyses where 1st order kinetics are desired.
References:
Hofmann J, Sernetz M, (1983) "A kinetic study on the enzymatic hydrolysis of fluorescein diacetate and fluorescein-di-beta-D-galactopyranoside." Anal. Biochem. 131(1): 180-186
Rotman B, Zderic JA, Edelstein M, (1963) "Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from flurescein (3,6-dihydroxyfluoran) and its monomethylether." Proc. Natl. Acad. Sci. USA 50: 1-6.
Huang Z, (1991) "Kinetic assay of fluorescein mono-b -D-galactoside hydrolysis by b -galactosidase: a front-face measurement for strongly absorbing fluorogenic substrates." Biochemistry 30(35): 8530-8534.
Burke BJ, Regnier FE, (2001) "Electrophoretically mediated microanalysis of b -galactosidase on microchips." Electrophoresis 22(17): 3744-3751.
Burke BJ, Regnier FE, (2003),"Stopped-Flow Enzyme Assays on a Chip Using a Microfabricated Mixer." Analytical Chemistry 75(8): 1786-1791.
Xu H, Ewing AG, (2004) "A rapid enzyme assay for b -galactosidase using optically gated sample introduction on a microfabricated chip." Analytical and Bioanalytical Chemistry 378(7): 1710-1715.
Park SS, Cho SI, Kim MS, Kim YK, Kim BG (2003) "Integration of on-column immobilized enzyme reactor in microchip electrophoresis." Electrophoresis 24(1-2): 200-206.
Okhonin V, Liu X, Krylov SN, (2005) "Transverse Diffusion of Laminar Flow Profiles To Produce Capillary Nanoreactors." Analytical Chemistry 77(18): 5925-5929.
Keywords:
Fluorescein, mono-β-D-Galactopyranoside, FMGal, Galactopyranoside, Marker Gene
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