Molecular Weight: 520.4
Absorption: (in nm) 505
Emission: (in nm) 535
Molecular Formula: C27H20O11
CAS Number: [85138-49-4]
Unit Price (USD):
10 mg $86.88
Bulk Price/unit (when you buy 5 or more): $69.50
Description:
Widely used fluorescent pH indicator for cellular and in vitro applications. This AM-ester derivative is membrane permeable and useful for staining live cells.
References:
"In vivo application of intestinal pH measurement Using 2,7-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence imaging." Marechal X, Mordon S, Devoisselle JM, Begu S, Guery B, Neviere R, Buys B, Dhelin G, Lesage JC, Mathieu D, Chopin C. Photochem. Photobiol. 70(5): 813-819 (1999)."BCECF in single cultured cells: inhomogeneous distribution but homogeneous response." Weinlich M, Theiss C, Lin CT, Kinne RK. J Exp. Biol. 201: 57-62 (1998). "Measurement of intracellular pH in fungal hyphae Using BCECF and digital imaging microscopy." Davies JM, Brownlee C, Jennings DH. J Cell. Sci. 96: 731 (1990). "Measurement of cytotoxicity by target cell release and retention of the fluorescent dye bis-carboxyethyl-carboxyfluorescein (BCECF)" Kolber MA, Quinones RR, Gress RE, Henkart PA. J Immunol. Meth. 108(1-2): 255-264 (1988)."Estimation of intramitochondrial pCa and pH by fura-2 and 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) fluorescence." Davis MH, Altschuld RA, Jung DW, Brierley GP. Biochem. Biophys. Res. Commun. 149(1): 40-45 (1987).
Keywords:
bis, Carboxyethyl, Carboxyfluorescein, BCECF, Marker Gene
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