Molecular Weight: 575.75

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Absorption: (in nm) 460

Emission: (in nm) 534

Molecular Formula: C₃₀H₄₉N₅O₆

CAS Number: [86701-10-2]

Unit Price (USD): 1 mg $140.00

Bulk Price/unit (when you buy 5 or more): $112.00

Description:
NBD-ceramide analog used to detect, at the cellular level, a variety of lysosomal storage diseases as well as intracellular distribution and transport of the labeled sphingolipid molecules in living cells.

Application:
Ceramides are the biological building blocks of more complex sphingolipids. Metabolism of ceramides typically occurs in Golgi and endoplasmic reticulum membranes.

References:

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• "Selective accumulation of the endoplasmic reticulum-golgi intermediate compartment induced by the antitumor drug KRN5500." Kamishohara M, Kenney S, Domergue R, Vistica DT, Sausville EA. Exp. Cell Res. 256(2): 468-479 (2000).
• "Involvement of sphingosine in mitochondria-dependent Fas-induced apoptosis of type II jurkat T cells." Cuvillier O, Edsall L, Spiegel S. J Biol. Chem. 275: 15691 (2000).
• "Defining lipid transport pathways in animal cells." Pagano RE, Sleight RG. Science 229: 1051 (1985).
• "A vital stain for the golgi apparatus." Lipsky NG, Pagano RE. Science 228: 745 (1985).
• "Intracellular translocation of fluorescent sphingolipids in cultured fibroblasts: endogenously synthesized sphingomyelin and glucocerebroside analogues pass through the golgi apparatus en route to the plasma membrane." Lipsky NG, Pagano RE. J Cell Biol. 100: 27 (1985).
• "Intracellular processing of lipids: a theory based on studies with fluorescent lipids, liposomes, and cells." Pagano RE. Liposome Letters, A.D. Bangham, Ed., pp. 83-96 (1983).