Molecular Weight: 338.3

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: H₂O, DMSO

Absorption: (in nm) 316

Emission: (in nm) 376

Extinction (10³): 14

Molecular Formula: C₁₆H₁₈O₈

CAS Number: [6160-78-7]

Unit Price (USD): 1 g $50.63

Bulk Price/unit (when you buy 5 or more): $40.50

Description:
Bright blue fluorogenic β-galactosidase substrate, especially useful for in vitro assays.

Note:
Absorbance 360nm/ Emission 449nm / E 19K of product 4-methylumbelliferone after enzyme hydrolysis.

References:

• "A microplate fluorimetric assay for transfection of the beta-galactosidase reporter gene." Rakhmanova VA, MacDonald RC. Anal. Biochem. 257: 234-237 (1998).
• "Human lysosomal beta-galactosidase-cathepsin A complex: definition of the beta-galactosidase-binding interface on cathepsin A." Pshezhetsky AV, Elsliger MA, Vinogradova MV, Potier M. Biochemistry 34: 2431-2440 (1995).
• "Highly sensitive fluorimetric enzyme immunoassay for prostaglandin H synthase solubilized from cultured cells." Ruan KH, Kulmacz RJ, Wilson A, Wu KK. J Immunol. Methods 162: 23-30 (1993).
• "Selective inactivation of eukaryotic beta-galactosidase in assays for inhibitors of HIV-1 TAT using bacterial beta-galactosidase as a reporter enzyme." Young DC, Kingsley SD, Ryan KA, Dutko FJ. Anal. Biochem. 215: 24-30 (1993).
• "Activation of cAMP-responsive genes by stimuli that produce long-term facilitation in Aplysia sensory neurons." Kaang BK, Kandel ER, Grant SG. Neuron 10: 427-435 (1993).
• "Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer." Price J, Turner D, Cepko C. Proc. Natl. Acad. Sci. USA 84: 156-160 (1987).