Molecular Weight: 656.6
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO, DMF, ETOH, H2O
Absorption: (in nm) 300
Molecular Formula: C32H32O15
CAS Number: [17817-20-8]
Alternative Name: Fluorescein di-galactoside; FDG
Unit Price (USD):
5 mg $86.88
Bulk Price/unit (when you buy 5 or more): $69.50
Description:
Ultra-sensitive substrate for lacZ β-galactosidase detection in vivo and in vitro.
Note:
Emission (EX: 488nm / EM: 512nm) of the fluorescein fluorophore released upon beta-Galactosidase enzyme activity.
Molecular Biology grade: <20 ppm background fluorescence QC specification.
Application:
See references below for numerous applications of this widely used substrate.
References:
Yeh JR, Zhang X, Nagano MC. (2011) “Wnt5a is a cell-extrinsic factor that supports selfrenewal of mouse spermatogonial stem cells.” J Cell Sci 124: 2357-2366.
Guo W, Wu H, (2008) "Detection of lacZexpression by FACS-Gal analysis" Nature Protocols DOI: 10.1038/nprot.2008.163 http://www.natureprotocols.com/2008/08/06/detection_of_lacz_expression_b.php.
Trowbridge JJ, Xenocostas A, Moon RT, Bhatia M. (2006) "Glycogen synthase kinase-3 is an in vivo regulator of hematopoietic stem cell repopulation." Nature Medicine 12: 89-98.
Dreier J, Breitmaier EB, Gocke E, Apfel CM, Page MGP. (2002) "Direct influence of S9 liver homogenate on fluorescence signals: impact on practical applications in a bacterial genotoxicity assay." Mutation Research 513(1-2): 169-182.
Burke BJ, Regnier FE. (2001) "Electrophoretically mediated microanalysis of &beta:-galactosidase on microchips." Electrophoresis 22(17): 3744-3751.
Nirenberg S. (2000) "Photoablation of cells expressing β-galactosidase." Methods Mol. Biol. 135: 475-80.
Nelis H, Van Poucke S. (2000) "Enzymatic detection of coliforms and Escherichia coli within 4 hours." Water, Air, and Soil Pollution 123(1-4): 43-52.
Berg M, et al. (2000) "Enzymatic detection of coliforms and Escherichia coli within 4 hours." J Biomol Screen 5: 71.
Additional References
Keywords:
Fluorescein di-β-D-Galactopyranoside, FDG, Fluorescein, di-β-D-Galactopyranoside, di-Beta-D-Galactopyranoside, di-B-D-Galactopyranoside, Marker Gene
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