| |
CAS Number: 64664-99-9
Description:
Blue fluorogenic beta-galactosidase substrate that is well retained intracellularly. The fluorescence spectrum for the released 3-carboxyumbelliferone is pH-dependent.
Application:
Below the pKa (7.8), Abs. shifts to shorter wavelengths (325 to 340 nm) and fluorescence intensity decreases. These shifts may be used to measure compartmentalization of intracellular activity. Note:
Em = 445nm, ε = 32K is emission of fluorophore 3-carboxyumbelliferone released upon beta-galactosidase enzyme activity.
View Technical Information ▼
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Absorption: (in nm) 330
Emission: (in nm) 396
Extinction (103): 16
Molecular Formula: C16H16O10
References:
View References ▼
"Enhanced detection of b-galactosidase reporter activation is achieved by a reduction of hemoglobin content in tissue lysates." Nazarenko DA, Dertinger SD, Gasiewicz TA. BioTechniques 30(4): 776-777, 780-781 (2001). "Water soluble coumarin derivatives for use as fluorogenic enzyme substrates." Huff JB, Merchant BT, Mullen CR, Venkata SRT. PCT Int. Appl. WO 9946402 (1999). "Expression of the Salmonella virulence plasmid gene spvB in cultured macrophages and nonphagocytic cells." Fierer J, Eckmann L, Fang F, Pfeifer C, Finlay BB, Guiney D. Infect Immun 61: 5231-5236 (1993). "Reducing background interference activity in enzyme-label immunoassays." Armenta R, Gibbons I, Olson J. U.S. Patent US 4668620 (1987). "Homogeneous substrate-labeled fluorescent immunoassay for disopyramide in human serum: semi- and fully automated procedures." Johnson PK, Messenger LJ. Clin Chem 32: 378-381 (1986). "Improved sensitivity in homogeneous enzyme immunoassays using a fluorogenic macromolecular substrate: an assay for serum ferritin." Armenta R, Tarnowski T, Gibbons I, Ullman EF. Anal Biochem 146: 211-219 (1985). "Homogeneous substrate-labeled fluorescent immunoassay for carbamazepine." Li TM, Miller JE, Ward FE, Burd JF. Epilepsia 23: 391-398 (1982). "Serum theophylline determination by fluorescence polarization immunoassay utilizing an umbelliferone derivative as a fluorescent label." Li TM, Benovic JL, Burd JF. Anal Biochem 118: 102-107 (1981). "Homogeneous substrate-labeled fluorescent immunoassay for theophylline in serum." Li TM, Benovic JL, Buckler RT, Burd JF. Clin Chem 27: 22-26 (1981). "The homogeneous substrate-labeled fluorescent immunoassay." Burd JF. Methods Enzymol 74: Pt C, 79-87 (1981). "Substrate-labeled homogeneous fluorescent immunoassay for tobramycin, ASEAN." Boguslaski RC, Burd JF, Denning CE. J. Clin. Sci. 1(3): 215-19 (1980). "Substrate-labeled fluorescent immunoassay for phenobarbital." Krausz LM, Hitz JB, Buckler RT, Burd JF. Ther. Drug Monit. 2(3): 261-72 (1980).
Alternative Name: High Purity-Molecular Biology Grade
|
Unit Price (USD):
5 mg $119.63
Bulk Price/unit
(5 or more): $95.70
|
