Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Absorption: (in nm) 330

Emission: (in nm) 396

Extinction (10³): 16

Molecular Formula: C₁₆H₁₆O₁₀

CAS Number: [64664-99-9]

Alternative Name: High Purity-Molecular Biology Grade

Unit Price (USD): 5 mg $108.50

Bulk Price/unit (when you buy 5 or more): $86.80

Description:
Blue fluorogenic beta-galactosidase substrate that is well retained intracellularly. The fluorescence spectrum for the released 3-carboxyumbelliferone is pH-dependent.

Note:
Em = 445nm, ε = 32K is emission of fluorophore 3-carboxyumbelliferone released upon beta-galactosidase enzyme activity.

Application:
Below the pKa (7.8), Abs. shifts to shorter wavelengths (325 to 340 nm) and fluorescence intensity decreases. These shifts may be used to measure compartmentalization of intracellular activity.

References:

• "Enhanced detection of b-galactosidase reporter activation is achieved by a reduction of hemoglobin content in tissue lysates." Nazarenko DA, Dertinger SD, Gasiewicz TA. BioTechniques 30(4): 776-777, 780-781 (2001).

• "Water soluble coumarin derivatives for use as fluorogenic enzyme substrates." Huff JB, Merchant BT, Mullen CR, Venkata SRT. PCT Int. Appl. WO 9946402 (1999).

• "Expression of the Salmonella virulence plasmid gene spvB in cultured macrophages and nonphagocytic cells." Fierer J, Eckmann L, Fang F, Pfeifer C, Finlay BB, Guiney D. Infect Immun 61: 5231-5236 (1993).

• "Reducing background interference activity in enzyme-label immunoassays." Armenta R, Gibbons I, Olson J. U.S. Patent US 4668620 (1987).

• "Homogeneous substrate-labeled fluorescent immunoassay for disopyramide in human serum: semi- and fully automated procedures." Johnson PK, Messenger LJ. Clin Chem 32: 378-381 (1986).

• "Improved sensitivity in homogeneous enzyme immunoassays using a fluorogenic macromolecular substrate: an assay for serum ferritin." Armenta R, Tarnowski T, Gibbons I, Ullman EF. Anal Biochem 146: 211-219 (1985).

• "Homogeneous substrate-labeled fluorescent immunoassay for carbamazepine." Li TM, Miller JE, Ward FE, Burd JF. Epilepsia 23: 391-398 (1982).

• "Serum theophylline determination by fluorescence polarization immunoassay utilizing an umbelliferone derivative as a fluorescent label." Li TM, Benovic JL, Burd JF. Anal Biochem 118: 102-107 (1981).

• "Homogeneous substrate-labeled fluorescent immunoassay for theophylline in serum." Li TM, Benovic JL, Buckler RT, Burd JF. Clin Chem 27: 22-26 (1981).

• "The homogeneous substrate-labeled fluorescent immunoassay." Burd JF. Methods Enzymol 74: Pt C, 79-87 (1981).

• "Substrate-labeled homogeneous fluorescent immunoassay for tobramycin, ASEAN." Boguslaski RC, Burd JF, Denning CE. J. Clin. Sci. 1(3): 215-19 (1980).

• "Substrate-labeled fluorescent immunoassay for phenobarbital." Krausz LM, Hitz JB, Buckler RT, Burd JF. Ther. Drug Monit. 2(3): 261-72 (1980).