Molecular Weight: 975.46
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: CHCl3, DMSO, MeOH
Absorption: (in nm) 342
Emission: (in nm) 470
Extinction (103): 40
Molecular Formula: C65H98O6
CAS Number: [102807-51-2]
Unit Price (USD):
1 mg $114.62
Bulk Price/unit (when you buy 5 or more): $91.70
Description:
Useful for measuring cholesteryl ester transfer and protein-mediated triacylglycerol transport between plasma lipoproteins. Has been found to be an excellent substrate for lipoprotein lipase and hepatic triacylglycerol lipase. See our kit M0612 for more information and protocols for use.
Note:
Em=377nm is the emission of the pyrenedecanoic acid monomer released upon lipase enzyme activity.
Application:
The fluorescent triacylglycerol 1,2-dioleoyl-3-(1-pyren-1-yl)decanoyl-rac-glycerol includes a pyrenedecanoic acid as one of the three fatty acyl residues of a natural triacylglycerol which forms excimers in vivo. Upon enzyme activity, the pyrenedecanoic acid monomer is cleaved, resulting in decreased excimer fluorescence.
References:
"Cholesteryl ester transfer protein reaction between plasma lipoproteins." Main LA, Okumura-Noji K, Ohnishi T, Yokoyama S. J Biochem (Tokyo) 124: 237-243 (1998)."Spectrofluorometric determination of lipase activity." Liodakis A, Drew J, Chan RY, Sawyer WH. Biochem. Int.23(5): 825-34 (1991)."Topo-dynamic characteristics of human plasma VLDL apolipoproteins and efficiency of triacylglycerol hydrolysis by lipoprotein lipase." Dergunov AD, Shuvaev VV, Perova NV. Biochim. Biophys. Acta 1005(1): 79-86 (1989)."Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase." Dousset N, Negre A, Salvayre R, Rogalle P, Dang QQ, Douste-Blazy L. Lipids 23: 605-608 (1988)."Transversal mobility in bilayer membrane vesicles: use of pyrene lecithin as optical probe." Galla HJ, Theilen U, Hartmann W. Chem. Phys. Lipids 23: 239-251 (1979)."Salvayre R., Negre, A., Radom, J., Douste-Blazy, L., (1986) "Fluorometric Assay for Pancreatic Lipase" CLIN. CHEM. 32(8): 1532-1536.
Keywords:
Dioleoyl, decanoyl- rac-Glycerol, decanoyl, Glycerol, Marker Gene
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