CAS Number: [N/A]
Unit Price (USD):
1 kit $112.50
Bulk Price/unit (when you buy 5 or more): $90.00
Description:
The Membrane Fluidity Kit allows quantitative monitoring of membrane fluidity in cell membranes, micelles, and vesicles through use of a lipophilic pyrene probe.
Application:
The dynamic properties of the cell membrane and cytoplasmic microtubules and microfilaments, as well as the dynamic movement of lipids in micelles and vesicles is of importance in such diverse areas as activation of polymorphonuclear leukocytes and chemotaxis, activation of membrane enzyme systems and the specific assembly or mobilization of microtubules and microfilaments, enhancement of the affinity of chemoattractant receptors, as well as being associated with a variety of pathological syndromes related to membrane fluidity.
It has been recognized that the rotational mobility of fluorescent or magnetic resonant probes is different from that observed in lateral diffusion. Membrane fluidity or "membrane viscosity" for short range lateral diffusion has best been measured using lipid analog probes that, when interacting, exhibit changes in their spectral properties. One of the best systems for use in such studies are the lipophilic pyrene probes that undergo eximer formation upon spatial interaction. When eximers form, the emission spectrum of the pyrene probe shifts dramatically to the red (longer wavelength). By measuring the ratio of monomer (EM max. 372 nm) to eximer (EM 470 nm) fluorescence, a quantitative monitoring of the membrane fluidity can be attained. These measurements can provide kinetic information, as well as in vivo monitoring of cellular function by both flow cytometry and microscopic analysis.
References:
"Pyrene eximer mapping in cultured fibroblasts by ratio imaging and time-resolved microscopy." Dix JA, Verkman AS. Biochemistry 29(7): 1949-1953 (1990).
"Measurement of membrane fluidity of polymorphonuclear leukocytes by flow cytometry." Masuda M, Kuriki H, Komiyama Y, Nishikado H, Egawa H, Murata K. J. Immunol. Meth. 96(2): 225-231 (1987).
"Membrane fluid properties of cord blood mononuclear leucocytes: association with increased insulin receptors." Neufeld ND, Corbo LM. Pediatric Res. 18(8): 773-778 (1984).
"Effect of membrane fluidizers on the number and affinity of chemotactic factor receptors on human polymorphonuclear leukocytes." Tomonaga A, Hirota M, Snyderman R. Microbiol. Immunol. 27(11): 961-972 (1983).
"Chemoattractant receptor functions in human polymorphonuclear leukocytes are divergently altered by membrane fluidizers." Yuli I, Tomonaga A, Synderman R. Proc. Natl. Acad. Sci. USA 79(19): 5906-5910 (1982).
"Membrane fluidity in human and mouse Chediak-Higashi leukocytes." Haak RA, Ingraham LM, Baehner RL, Boxer LA. J. Clin Invest. 64(1): 138-144 (1979).
"Cell biology of leukocyte abnormalities-membrane and cytoskeletal function in normal and defective cells. A review." Oliver JM. Amer. J. Pathology 93(1): 221-270 (1978).
Keywords:
Membrane Fluidity Kit, Membrane, Fluidity, Kit, lipophilic pyrene, lipophilic, pyrene, Marker Gene 
