Molecular Weight: 331.33

Storage: RT, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: H₂O, DMSO

Absorption: (in nm) 494

Emission: (in nm) 521

Extinction (10³): 73

Molecular Formula: C₂₀H₁₃NO₄

CAS Number: [3086-44-0]

Unit Price (USD): 5 mg $76.45

Bulk Price/unit (when you buy 5 or more): $61.16

Description:
Highly fluorescent dye useful for oligonucleotide, antibody and protein labeling studies and for the preparation of peptide and esterase substrates.

Application:
Rhodol is a structural hybrid of fluorescein and rhodamine. It retains the pH-sensitive properties conferred by a phenolic hydroxyl group, but the amine substituent reduces the pKa values. Rhodol exhibits a high extinction coefficient (~70,000 cm-1M-1) and high quantum yield (~0.8-0.9) in aqueous solution, with absorption maxima at approximately 490 nm and emission maxima between 520 and 545 nm. As with fluorescein-based dyes, the fluorescence excitation profiles of rhodol-based dyes are pH dependent, which can be useful for ratiometric pH determinations intracellularly. See the references below or call us for more information about your labeling protocol.

References:

• "Stains, labels and detection strategies for nucleic acids assays." Kricka, L.J., Ann. Clin. Biochem. (2002)39:114-29

• "Fluorescent rhodol derivatives: versatile, photostable labels and tracers." Whitaker,J.E., Haugland, R.P., Ryan, D., Hewitt, P.C., Haugland, R.P., Prendergast, F.G., Anal. Biochem. (1992)207:267-279.

• "Rhodamine dyes and related compounds. XII. Diacetyl derivatives of rhodamine and rhodol; structure of colorless forms of fluoran dyes." Ioffe, I. S., Otten, V. F., Zh. Organ. Khim. (1965)1(2):336-9.

• "Rhodamine dyes and related compounds. II. Rhodol, its preparation and properties.", Ioffe, I. S., Otten, V. F., Zh. Obshch. Khim.,(1962)32:1196-201.

• "Fluorescein Substitutes for Microscopy and Imaging." Haugland, R.P., Optical Microscopy for Biology, Herman. B., Jacobson, K., Eds. pp. 143-157 (1990).