Molecular Weight: 380.83

Storage: RT

Soluble: DMSO, DMF, MeOH

Absorption: (in nm) 505

Emission: (in nm) 534

Molecular Formula: C₂₁H₁₇N₂O₃Cl

CAS Number: [62669-70-9]

Unit Price (USD): 25 mg $50.63

Bulk Price/unit (when you buy 5 or more): $40.50

Description:
This cell-permeant, green fluorescent dye is used in many applications including bacterial viability and vitality assays, mitochondrial membrane potential assays in apoptotic cells, and sorting respiratory-deficient yeast cells using flow cytometry.

References:

• "Rapid Assessment of Bacterial Viability and Vitality by Rhodamine 123 and Flow Cytometry." Kaprelyants AS, Kell DB. J Appl Bacteriol 72: 410 (1992).
• "Staining of Living Bacteria with Rhodamine 123." Matsuyama T. FEMS Microbiol Lett 21: 153 (1984).
• "Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells." Skowronek P, Krummeck G, Haferkamp O, Rodel G. Curr Genet 18: 265-267 (1990).
• "Temperature-sensitive yeast mutants defective in mitochondrial inheritance." McConnell SJ, Stewart LC, Talin A, Yaffe MP. J Cell Biol 111: 967-976 (1990).
• "Intracellular ionic variations in the apoptotic death of L cells by inhibitors of cell cycle progression." Barbiero G, Duranti F, Bonelli G, Amenta JS, Baccino FM. Exp Cell Res 217: 410-418 (1995).
• "Features of apoptotic cells measured by flow cytometry." Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. Cytometry 13: 795-808 (1992).
• "Mitochondrial modifications during rat thymocyte apoptosis: a study at the single cell level." Cossarizza A, Kalashnikova G, Grassilli E, Chiappelli F, Salvioli S, Capri M, Barbieri D, Troiano L, Monti D, Franceschi C. Exp Cell Res 214: 323-330 (1994).
• "Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor." Deckwerth TL, Johnson EM Jr. J Cell Biol 123: 1207-1222 (1993).
• "Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives." Scaduto RC Jr, Grotyohann LW. Biophys J 76: 469-477 (1999).
• "A fast kinetic method for assessing mitochondrial membrane potential in isolated hepatocytes with rhodamine 123 and flow cytometry." Juan G, Cavazzoni M, Saez GT, OConnor JE. Cytometry 5: 335-342 (1994).
• "Flow Cytometric Analysis of Rhodamine 123 Fluorescence during Modulation of the Membrane Potential in Plant Mitochondria." Petit PX. Plant Physiol 98: 279 (1992).
• "Localization of mitochondria in living cells with rhodamine 123." Johnson LV, Walsh ML, Chen LB. Proc Natl Acad Sci USA 77: 990-994 (1980).
• "Scanning microspectrofluorometry of rhodamine 123 in multidrug-resistant cells." Millot JM, Sharonov S, Manfait M. Cytometry 17(1): 50-58 (1994).