Molecular Weight: 380.83
Storage: RT
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO, DMF, MeOH
Absorption: (in nm) 505
Emission: (in nm) 534
Molecular Formula: C21H17N2O3Cl
CAS Number: [62669-70-9]
Unit Price (USD):
25 mg $53.16
Bulk Price/unit (when you buy 5 or more): $42.52
Description:
Cell-permeant, green fluorescent dye is used in many applications including bacterial viability and vitality assays, mitochondrial membrane potential assays in apoptotic cells, and sorting respiratory-deficient yeast cells using flow cytometry.
References:
- "Rapid Assessment of Bacterial Viability and Vitality by Rhodamine 123 and Flow Cytometry." Kaprelyants AS, Kell DB. J Appl Bacteriol 72: 410 (1992).
- "Staining of Living Bacteria with Rhodamine 123." Matsuyama T. FEMS Microbiol Lett 21: 153 (1984).
- "Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells." Skowronek P, Krummeck G, Haferkamp O, Rodel G. Curr Genet 18: 265-267 (1990).
- "Temperature-sensitive yeast mutants defective in mitochondrial inheritance." McConnell SJ, Stewart LC, Talin A, Yaffe MP. J Cell Biol 111: 967-976 (1990).
- "Intracellular ionic variations in the apoptotic death of L cells by inhibitors of cell cycle progression." Barbiero G, Duranti F, Bonelli G, Amenta JS, Baccino FM. Exp Cell Res 217: 410-418 (1995).
- "Features of apoptotic cells measured by flow cytometry." Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. Cytometry 13: 795-808 (1992).
- "Mitochondrial modifications during rat thymocyte apoptosis: a study at the single cell level." Cossarizza A, Kalashnikova G, Grassilli E, Chiappelli F, Salvioli S, Capri M, Barbieri D, Troiano L, Monti D, Franceschi C. Exp Cell Res 214: 323-330 (1994).
- "Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor." Deckwerth TL, Johnson EM Jr. J Cell Biol 123: 1207-1222 (1993).
- "Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives." Scaduto RC Jr, Grotyohann LW. Biophys J 76: 469-477 (1999).
- "A fast kinetic method for assessing mitochondrial membrane potential in isolated hepatocytes with rhodamine 123 and flow cytometry." Juan G, Cavazzoni M, Saez GT, OConnor JE. Cytometry 5: 335-342 (1994).
- "Flow Cytometric Analysis of Rhodamine 123 Fluorescence during Modulation of the Membrane Potential in Plant Mitochondria." Petit PX. Plant Physiol 98: 279 (1992).
- "Localization of mitochondria in living cells with rhodamine 123." Johnson LV, Walsh ML, Chen LB. Proc Natl Acad Sci USA 77: 990-994 (1980).
- "Scanning microspectrofluorometry of rhodamine 123 in multidrug-resistant cells." Millot JM, Sharonov S, Manfait M. Cytometry 17(1): 50-58 (1994).
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