MGT Inc. Login  |  Register  |  Newsletter Subscription  |  Cart 
MGT Inc.
MGT Inc.
MGT Inc.
    Home         Products         About Us/Contact         Research         Jobs         Newsletter    
     

Products


Vectors Vectors
Enzyme Substrates and Kits Enzyme Substrates and Kits
Enzyme Inhibitors Enzyme Inhibitors
MGT Detection Kits MGT Detection Kits
Cell Regulatory Substrates Cell Regulatory Substrates
Special Labeling Reagents Special Labeling Reagents
Lipids Lipids
Carbohydrates/Oligosaccharides Carbohydrates/Oligosaccharides  
Fluorescent Reagents Fluorescent Reagents

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Haiti Relief FundDuring the month of February, Marker Gene will donate 10% from the revenues of all orders placed online at www.markergene.com to the Save the Children’s Support the Haiti Earthquake Children in Emergency Fund. Thanks for your help!
M0545 - Dihydrorhodamine 123 (DHR123)

Molecular Weight: 346.39

Storage: F, D, L

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO, CHCl3, MeOH

Absorption: (in nm) 300

Molecular Formula: C21H18N2O3

CAS Number: [109244-58-8]

Unit Price (USD): 2 mg $43.38

Bulk Price/unit (when you buy 5 or more): $34.70

Description:
One of the most widely used Reactive Oxygen Species (ROS) probes for intracellular measurement and analysis.

Note:
Cleavage product: Upon enzymatic (peroxidase) reaction or chemical oxidation, the brightly fluorescent Rhodamine 123 is produced (EX: 505 nm / EM: 534 nm, e=98K/mole).

References:

  • "Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes." G. Rothe, A. Oser, G. Valet Naturwissenschaften 75: 354-355 (1988).
  • "A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123." A. Emmendorffer, M. Hecht, M.L. Lohmann-Matthes, J. Roesler, J Immunol. Methods 131: 269-275 (1990).
  • "Flow cytometric measurement of the respiratory burst activity of phagocytes using dihydrorhodamine 123." G. Rothe, A. Emmendorffer, A. Oser, J. Roesler, G. Valet, J. Immunol. Methods 138: 133-135 (1991).
  • "The oxidative metabolism of glutamine. A modulator of reactive oxygen intermediate-mediated cytotoxicity of tumor necrosis factor in L929 fibrosarcoma cells." V. Goossens, J. Grooten, W. Fiers, J. Biol. Chem. 271: 192-196 (1996).
  • "Dihydrorhodamine 123 identifies impaired mitochondrial respiratory chain function in cultured cells harboring mitochondrial DNA mutations." C. Sobreira, M. Davidson, M.P. King, A.F. Miranda, J. Histochem. Cytochem. 44: 571-579 (1996).
  • "The regulation of reactive oxygen species production during programmed cell death." S. Tan, Y. Sagara, Y. Liu, P. Maher, D. Schubert, J. Cell Biol. 141: 1423-1432 (1998).
  • "A sensitive flow cytometric method for measuring the oxidative burst." M.A. Model, M.A. KuKuruga, R.F. Todd, J. Immunol. Methods 202: 105-111 (1997).
  • "Identification of oxidant-sensitive proteins: TNF-alpha induces protein glutathiolation." D.M. Sullivan, N.B. Wehr, N.M. Fergusson, R.L. Levine, T. Finkel, Biochemistry 39: 11121-11128 (2000).



    • Keywords:

    Dihydrorhodamine, DHR123, ROS, Marker Gene


    ©2010 Marker Gene Technologies, Inc. Published by Marker Gene Technologies, Inc., The University of Oregon Riverfront Research Park, 1850 Millrace Drive, Eugene, Oregon 97403-1992 USA. All rights reserved. For information on the use or copying of the material contained in this document, please contact us at techservice@markergene.com.