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Haiti Relief FundDuring the month of February, Marker Gene will donate 10% from the revenues of all orders placed online at www.markergene.com to the Save the Children’s Support the Haiti Earthquake Children in Emergency Fund. Thanks for your help!

M0545 - Dihydrorhodamine 123 (DHR123)

Molecular Weight: 346.39

Storage: F, D, L

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO, CHCl3, MeOH

Absorption: (in nm) 300

Molecular Formula: C21H18N2O3

CAS Number: [109244-58-8]

Unit Price (USD): 2 mg $43.38

Bulk Price/unit (when you buy 5 or more): $34.70

Description:
One of the most widely used Reactive Oxygen Species (ROS) probes for intracellular measurement and analysis.

Note:
Cleavage product: Upon enzymatic (peroxidase) reaction or chemical oxidation, the brightly fluorescent Rhodamine 123 is produced (EX: 505 nm / EM: 534 nm, e=98K/mole).

References:

  • "Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes." G. Rothe, A. Oser, G. Valet Naturwissenschaften 75: 354-355 (1988).
  • "A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123." A. Emmendorffer, M. Hecht, M.L. Lohmann-Matthes, J. Roesler, J Immunol. Methods 131: 269-275 (1990).
  • "Flow cytometric measurement of the respiratory burst activity of phagocytes using dihydrorhodamine 123." G. Rothe, A. Emmendorffer, A. Oser, J. Roesler, G. Valet, J. Immunol. Methods 138: 133-135 (1991).
  • "The oxidative metabolism of glutamine. A modulator of reactive oxygen intermediate-mediated cytotoxicity of tumor necrosis factor in L929 fibrosarcoma cells." V. Goossens, J. Grooten, W. Fiers, J. Biol. Chem. 271: 192-196 (1996).
  • "Dihydrorhodamine 123 identifies impaired mitochondrial respiratory chain function in cultured cells harboring mitochondrial DNA mutations." C. Sobreira, M. Davidson, M.P. King, A.F. Miranda, J. Histochem. Cytochem. 44: 571-579 (1996).
  • "The regulation of reactive oxygen species production during programmed cell death." S. Tan, Y. Sagara, Y. Liu, P. Maher, D. Schubert, J. Cell Biol. 141: 1423-1432 (1998).
  • "A sensitive flow cytometric method for measuring the oxidative burst." M.A. Model, M.A. KuKuruga, R.F. Todd, J. Immunol. Methods 202: 105-111 (1997).
  • "Identification of oxidant-sensitive proteins: TNF-alpha induces protein glutathiolation." D.M. Sullivan, N.B. Wehr, N.M. Fergusson, R.L. Levine, T. Finkel, Biochemistry 39: 11121-11128 (2000).


  • Keywords:

    Dihydrorhodamine, DHR123, ROS, Marker Gene


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