Molecular Weight: 497.83

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Molecular Formula: C₁₈H₂₄NO₁₃Cl

CAS Number: [120583-41-7]

Unit Price (USD): 25 mg $106.95

Bulk Price/unit (when you buy 5 or more): $85.56

Description:
Chromogenic substrate for lactosidase activity. The released product, 2-chloro-4-nitrophenol (CNP) is usually monitored spectrophotometrically at 405 nm. The rate of formation of 2-chloro-4-nitrophenol is proportional to the lactosidase activity in the sample. The pKa of CNP is approximately 5.5.

References:

• Nidetzky, Bernd; Claeyssens, Marc. Specific quantification of Trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies. Biotechnol. Bioeng. (1994), 44(8), 961-6.
• Claeyssens, Marc; Van Tilbeurgh, Herman; Tomme, Peter; Wood, Thomas M.; McRae, Sheila I. Fungal cellulase systems. Comparison of the specificities of the cellobiohydrolases isolated from Penicillium pinophilum and Trichoderma reesei. Biochem. J. (1989), 261(3), 819-25.
• Tomme, Peter; Claeyssens, Marc. Identification of a functionally important carboxyl group in cellobiohydrolase I from Trichoderma reesei. A chemical modification study. FEBS Lett. (1989), 243(2), 239-43.