Molecular Weight: 500.45

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Molecular Formula: C₂₂H₂₈O₁₃

CAS Number: [84325-23-5]

Unit Price (USD): 25 mg $72.38

Bulk Price/unit (when you buy 5 or more): $57.90

Description:
Fluorescent substrate for β-lactosidase or galactosidase and glucosidase activities; releases 4-methylumbelliferone (EX 385 nm, EM 502 nm, e = 16K) upon enzyme action. The fluorescence is optimal at pH values above 8-9.

References:

• Parry, Neil J.; Beever, David E.; Owen, Emyr; Vandenberghe, Isabel; Van Beeumen, Jozef; Bhat, Mahalingeshwara K. Biochemical characterization and mechanism of action of a thermostable b-glucosidase purified from Thermoascus aurantiacus. Biochemical Journal (2001), 353(1), 117-127.
• Miura, Yoshiaki; Kim, Soohyun; Etchison, James R.; Ding, Yili; Hindsgaul, Ole; Freeze, Hudson H. Aglycone structure influences a-fucosyltransferase III activity using N-acetyllactosamine glycoside acceptors. Glycoconjugate Journal (2000), Volume Date 1999, 16(11), 725-730.
• Minke, Wendy E.; Roach, Claudia; Hol, Wim G. J.; Verlinde, Christophe L. M. J. Structure-Based Exploration of the Ganglioside GM1 Binding Sites of Escherichia coli Heat-Labile Enterotoxin and Cholera Toxin for the Discovery of Receptor Antagonists. Biochemistry (1999), 38(18), 5684-5692.
• Miller, Morten; Palojarvi, Ansa; Rangger, Andrea; Reeslev, Morten; Kjoller, Annelise. The use of fluorogenic substrates to measure fungal presence and activity in soil. Applied and Environmental Microbiology (1998), 64(2), 613-617.
• Ahmed, Hafiz; Fink, Nilda E.; Pohl, Jan; Vasta, Gerardo R. Galectin-1 from bovine spleen: biochemical characterization, carbohydrate specificity and tissue-specific isoform profiles. Journal of Biochemistry (Tokyo) (1996), 120(5), 1007-1019.
• Wang, Lai-Xi; Pavlova, Nadejda V.; Li, Su-Chen; Li, Yu-Teh; Lee, Yuan C. A fluorometric assay of ceramide glycanase with 4-methylumbelliferyl b-D-lactoside derivatives. Glycoconjugate J. (1996), 13(3), 359-365.
• Claeyssens, Marc; Van Tilbeurgh, Herman; Tomme, Peter; Wood, Thomas M.; McRae, Sheila I. Fungal cellulase systems. Comparison of the specificities of the cellobiohydrolases isolated from Penicillium pinophilum and Trichoderma reesei. Biochem. J. (1989), 261(3), 819-25.
• Van Tilbeurgh, Herman; Claeyssens, Marc. Detection and differentiation of cellulase components using low molecular mass fluorogenic substrates. FEBS Lett. (1985), 187(2), 283-8.
• Claeyssens, Marc; Van Tilbeurgh, Herman; Kamerling, Johannis P.; Berg, Jan; Vrsanska, Maria; Biely, Peter. Studies of the cellulolytic system of the filamentous fungus Trichoderma reesei QM 9414. Substrate specificity and transfer activity of endoglucanase I. Biochem. J. (1990), 270(1), 251-6.
• Van Tilbeurgh, Herman; Claeyssens, Marc; De Bruyne, Clement K. The use of 4-methylumbelliferyl and other chromophoric glycosides in the study of cellulolytic enzymes. FEBS Lett. (1982), 149(1), 152-6.