Molecular Weight: 500.45

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Molecular Formula: C₂₂H₂₈O₁₃

CAS Number: [84325-23-5]

Unit Price (USD): 25 mg $83.24

Bulk Price/unit (when you buy 5 or more): $66.59

Description:
Fluorescent substrate for β-lactosidase or galactosidase and glucosidase activities; releases 4-methylumbelliferone (EX 385 nm, EM 502 nm, e = 16K) upon enzyme action. The fluorescence is optimal at pH values above 8-9.

References:

• Parry NJ, Beever DE, Owen E, Vandenberghe I, Van Beeumen J, Bhat MK. (2001) "Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus." Biochem J 353(1): 117-127.
• Miura Y, Kim S, Etchison JR, Ding Y, Hindsgaul O, Freeze HH. (2000) "Aglycone structure influences a-fucosyltransferase III activity using N-acetyllactosamine glycoside acceptors." Glycoconjugate J 16(11):725-730.
• Minke WE, Roach C, Hol WGJ, Verlinde CLMJ. (1999) "Structure-Based Exploration of the Ganglioside GM1 Binding Sites of Escherichia coli Heat-Labile Enterotoxin and Cholera Toxin for the Discovery of Receptor Antagonists." Biochem 38(18): 5684-5692.
• Miller M, Palojarvi A, Rangger A, Reeslev M, Kjoller A. (1998) "The use of fluorogenic substrates to measure fungal presence and activity in soil." Appl Envir Microbio 64(2): 613-617.
• Ahmed H, Fink NE, Pohl J, Vasta GR. (1996) "Galectin-1 from bovine spleen: biochemical characterization, carbohydrate specificity and tissue-specific isoform profiles." J of Biochem (Tokyo) 120(5): 1007-1019.
• Wang LX, Pavlova NV, Li SC, Li YT, Lee YC. (1996) "A fluorometric assay of ceramide glycanase with 4-methylumbelliferyl beta-D-lactoside derivatives." Glycoconjugate J 13(3): 359-365.
• Claeyssen M, Van Tilbeurgh H, Tomme P, Wood TM, McRae SI. (1989) "Fungal cellulase systems. Comparison of the specificities of the cellobiohydrolases isolated from Penicillium pinophilum and Trichoderma reesei." Biochem. J 261(3): 819-25.
• Van Tilbeurgh H, Claeyssens M. (1985) "Detection and differentiation of cellulase components using low molecular mass fluorogenic substrates." FEBS Lett. 187(2): 283-8.
• Claeyssens M, Van Tilbeurgh H, Kamerling JP, Berg J, Vrsanska M, Biely P. (1990) "Studies of the cellulolytic system of the filamentous fungus Trichoderma reesei QM 9414. Substrate specificity and transfer activity of endoglucanase I." Biochem J 270(1): 251-6.
• Van Tilbeurgh H, Claeyssens M, De Bruyne CK. (1982) "The use of 4-methylumbelliferyl and other chromophoric glycosides in the study of cellulolytic enzymes." FEBS Lett 149(1): 152-6.