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Fluorescent and Luminescent Tools for Life Science

MarkerGene™ Fluorescent Lipase Assay Kit (CAS [102807-51-2] for substrate reagent)

M0612
Application: Allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the fluorescent fatty acid substrate 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol M0258.

CAS Number: [102807-51-2] for substrate reagent


Ordering Information:
Product ID Unit Size Number of Units Price
M0612 1 1-4 $239.00
    5+ $191.20

 

Description

Lipases are a family of enzymes that release fatty acids from triacylglycerols in a site specific manner. Most lipases have optimum activity for the primary ester groups of triglycerides, while some lipases remove fatty acyl groups from either the C-1 or C-3 acyl positions. The substrate is typically not a single molecule, but a nonaqueous phase of aggregated lipid. Lipase activity, ubiquitous among most cells, can be monitored using the new fluorescent substrate 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol M0258 contained in the kit. Upon cleavage, the fluorescent fatty acid pyrenedecanoic acid M0274 is released and activity measurements are easily obtained either in vitro, in cell preparations, or in vivo. The kit contains enough substrate for numerous assays and control experiments, and also contains reference standards and a detailed protocol for use. See the references below for more information and applications.


References:

  • Main, L.A., Okumura-Noji, K., Ohnishi, T., Yokoyama, S. (1998) "Cholesteryl ester transfer protein reaction between plasma lipoproteins." J. Biochem. (Tokyo) 124: 237-243.
  • Jaeger, K.E., Ransac, S., Dijkstra, B.W., Colson, C., van Heuvel, M., Misset, O. (1994) "Bacterial Lipases" FEMS Microbiol. Rev. 15(1): 29-63.
  • Dousset, N., Negre, A., Salvayre, R., Rogalle, P., Dang, Q.Q., Douste-Blazy, L. (1988) "Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase." Lipids 23: 605-608.
  • Galla, H.J., Theilen, U., Hartmann, W. (1979) "Transversal Mobility in Bilayer Membrane Vesicles: Use of Pyrene Lecithin as Optical Probe" Chem. Phys. Lipids 23: 239-251.
  • Dergunov, A.,D., Shuvaev, V.V., Perova N.V. (1989) "Topo-dynamic characteristics of human plasma VLDL apolipoproteins and efficiency of triacylglycerol hydrolysis by lipoprotein lipase." Biochim. Biophys. Acta 1005(1): 79-86.
  • Liodakis, A., Drew, J., Chan, R.Y., Sawyer, W.H. (1991) "Spectrofluorometric determination of lipase activity." Biochem. Int. 23(5): 825-34.
  • Negre, A., Salvayre, R., Dousset, N., Rogalle, P., Dang, Q.Q., Douste-Blazy, L. (1988) "Hydrolysis of fluorescent pyrenetriacylglycerols by lipases from human stomach and gastric juice." Biochim. Biophys. Acta 963: 340-348.
  • Hendrickson, H.S. (1994) "Fluorescence-bases assays of lipases, phospholipases, and other lipolytic enzymes." Anal. Biochem. 219: 1-8.
  • Negre-Salvayre, A., Abouakil, N., Lombardo, D., Salvayre, R. (1990) "Hydrolysis of fluorescent pyrene-acyl esters by human pancreatic carboxylic ester hydrolase and bile salt-stimulated lipase." Lipids 25(8):428-434.
  • Negre, A., Maret, A., Douste-Blazy, L., Gatt, S., Salvayre, R. (1988) "Relative fluorescence of normal and acid lipase-deficient cultured fibroblasts following administration of pyrene decanoic acid." Biochim. Biophys. Acta 960: 401-409.
  • Negre, A., Salvayre, R., Dagan, A., Gatt, S. (1985) "New fluorometric assay of lysosomal acid lipase and its application to the diagnosis of Wolman and cholesteryl ester storage diseases." Clin. Chim. Acta 149: 81-88.
  • Rosseneu, M., Taveirne, M., Caster, H., Van Biervliet, J. (1985) "Hydrolysis of very-low-density lipoproteins labeled with a fluorescent triacylglycerol: 1,3-dioleoyl-2-(4-pyrenylbutanoyl)glycerol." Eur. J. Biochem. 152: 195-198.
  • Mantulin, W.M., Massey, J.B., Gotto, A.M., Pownall, H.J. (1981) "Reassembled Model Lipoproteins." J. Biol. Chem. 256(21): 10815-10819.
  • Liodakis, A., Drew, J., Chan, R., Sawyer, W.H. (1991) "Spectrofluorometric determination of lipase activity." Biochem. Intern. 23(5): 825-834.
  • Jaeger K.E., Ransac S., Dijkstra B.W., Colson C., van Heuvel M., Misset O., 1994."Bacterial lipases." FEMS Microbiol Rev. 15(1):29-63.
  • Arpigny J. L., Jaeger K.-E., "Bacterial lipolytic enzymes: classification and properties. (1999) " Biochem. J. 343: 177-183.
  • Kouker G., Jaeger K.E., (1987) "Specific and sensitive plate assay for bacterial lipases." Appl Environ Microbiol) 53(1): 211-3.
  • Howard G.T., Vicknair J., MacKie R.I., (2001) "Sensitive plate assay for screening and detection of bacterial polyurethanase activity." Lett. Appl. Microbiol. 32(3): 211-4.
  • Salvayre R., Negre, A., Radom, J., Douste-Blazy, L., (1986) "Fluorometric Assay for Pancreatic Lipase" Clin. Chem. 32(8): 1532-1536.
  • Low KL, Srinivasa Rao PS, Shui G, Bendt AK, Pethe K, Dick T, Wenk MR. (2009) “Triacylglycerol utilization is required for regrowth of in vitro hypoxic nonreplicating Mycobacterium bovis bacillus Calmette-Guerin.” J Bacter 191: 5037-5043.
  • Millar JS, Duffy D, Gadi R, Bloedon LT, Dunbar RL, Wolfe ML, Movva R, Shah A, Fuki IV, McCoy M, Harris CJ, Wang MD, Howey DC, Rader DJ. (2009) “Potent and selective PPAR-α agonist LY518674 upregulates both ApoA-I production and catabolism in human subjects with the metabolic syndrome.” Arterioscler Thromb Vasc Biol 29:140-146.