Fluorescent and Luminescent Tools for Life Science

MarkerGene™ Live Cell Luciferase Assay Kit

M0626
Application: Contains synthetic D-luciferin, the primary substrate of the firefly luciferase light producing system, as well as a luciferase assay buffer


Ordering Information:
Product ID Unit Size Number of Units Price
M0626 1 1-4 $111.91
    5+ $89.53

 

Description

This kit will perform 1000 assays using a microtiterplate format (100 uL reaction volume, 0.1 mM final reaction concentration). The synthetic beetle luciferin substrate contained in the kit is also available separately as a lyophilized powder, D-Luciferin M0237. Consult the Product information sheet (above) for more details on the assay protocol.


References:

  • "Transmembrane Motility Assay of Transiently Transfected Cells by Fluorescent Cell Counting and Luciferase Measurement." J.J. Gildea, et al. Biotechniques 29: 81 (2000).
  • "The Use of the Luciferase Reporter System in Plant Gene Expression Studies." W. Van Leeuwen, et al. Plant Mol. Biol. Rep. 18:143a (2000).
  • "Reporter Genes and Transient Assays for Plants." B.F. Matthews, et al. Methods Mol. Biol. 55:147 (1995)
  • "Firefly Luciferase, Synthesized to Very High Levels in Caterpillars Infected with a Recombinant Baculovirus, Can Also Be Used As an Effective Reporter Enzyme In Vivo." P.K. Jha, et al. FEBS Lett. 274: 23 (1990)