Molecular Formula: N/A
Unit Price (USD):
1 kit $106.58
Bulk Price/unit (when you buy 5 or more): $85.26
Description:
Contains synthetic D-luciferin, the primary substrate of the firefly luciferase light producing system, as well as a luciferase assay buffer
Application:
This kit will perform 1000 assays using a microtiterplate format (100 uL reaction volume, 0.1 mM final reaction concentration). The synthetic beetle luciferin substrate contained in the kit is also available separately as a lyophilized powder, D-Luciferin M0237. Consult the Product information sheet (above) for more details on the assay protocol.
References:
"Transmembrane Motility Assay of Transiently Transfected Cells by
Fluorescent Cell Counting and Luciferase Measurement." J.J. Gildea, et al.
Biotechniques 29: 81 (2000).
"The Use of the Luciferase Reporter System in Plant Gene Expression
Studies." W. Van Leeuwen, et al. Plant Mol. Biol. Rep. 18:143a (2000).
"Reporter Genes and Transient Assays for Plants." B.F. Matthews, et al.
Methods Mol. Biol. 55:147 (1995)
"Firefly Luciferase, Synthesized to Very High Levels in Caterpillars Infected
with a Recombinant Baculovirus, Can Also Be Used As an Effective
Reporter Enzyme In Vivo." P.K. Jha, et al. FEBS Lett. 274: 23 (1990)
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