Fluorescent and Luminescent Tools for Life Science

5-(Iodoacetamido)fluorescein (CAS 63368-54-7)

M0638
Application: Excellent thiol reactive fluorescein derivative for synthesis of fluorescently labeled organelles, proteins and peptides at or near their physiological pH values (pH 7) including enzymes (e.g. (Na, K)-ATPase) or organelles like nucleii and nuclear matrices. Labels thiol groups on proteins specifically and efficiently.

CAS Number: 63368-54-7

Molecular Weight: 515.3

Molecular Formula: C22H14INO6


Ordering Information:
Product ID Unit Size Number of Units Price
M0638 25 mg 1-4 $69.92
    5+ $55.94

 

Note: Ex=490-496 nm


Technical Information

Storage: F, D, L

Soluble: DMSO, DMF, water (pH six+)

Emission: (in nm) 520

Extinction (103): 75


References:

  • "Use of a distant reporter group as evidence for a conformational change in a sensory receptor." Zukin, R.S., Hartig, P.R., Koshland, D.E. Jr., Proc. Natl. Acad. Sci. USA 74:1932-1936 (1977).
  • "Specific chemical labeling of DNA fragments." Eshaghpour, H., Soll, D., Crothers, D.M., Nucleic Acids Res. 7:1485-1495 (1979).
  • "Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin." Wang, Y.L., Taylor, D.L., J. Histochem. Cytochem. 28:1198-1206 (1980).
  • "Use of fluorescence resonance energy transfer to investigate the conformation of DNA substrates bound to the Klenow fragment." Furey, W.S., Joyce, C.M., Osborne, M.A., Klenerman, D., Peliska, J.A., Balasubramanian, S., Biochemistry 37:2979-2990 (1998).
  • "Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria." Schofield, P., Pell, A.N., Krause, D.O., Appl. Environ. Microbiol. 63:1143-1147(1997).
  • "Design and characterization of a multisite fluorescence energy-transfer system for protein folding studies: a steady-state and time-resolved study of yeast phosphoglycerate kinase." Lillo, M.P., Beechem, J.M., Szpikowska, B.K., Sherman, M.A., Mas, M.T., Biochemistry 36:11261-11272 (1997).
  • "An equivalent site mechanism for Na+ and K+ binding to sodium pump and control of the conformational change reported by fluorescein 5-isothiocyanate modification." Smirnova, I.N., Lin, S.H., Faller, L.D., Biochemistry 34:8657-8667 (1995).
  • "Diffusion coefficient of the cyclic GMP analog 8-(fluoresceinyl)thioguanosine 3,5 cyclic monophosphate in the salamander rod outer segment." Koutalos, Y., Brown, R.L., Karpen, J.W., Yau, K.W., Biophys. J. 69:2163-2167 (1995).
  • Kapakos, JG, Steinberg M, (1986) "5-Iodoacetamidofluorescein-labeled (Na,K)-ATPase. Steady-state fluorescence during turnover." J. Biol. Chem. 261(5):2090-2095.
  • Martelli AM, Billi AM, De Marchis C, Manzoli L, Cocco L. (1990) "Probing the sulfhydryl groups of nuclear matrix proteins with 6-iodoacetamidofluorescein."Cell Biol. Int. Rep. 14(5):409-18.