Molecular Weight: 410.81
Storage: F, D, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO, DMF, water (pH six+)
Absorption: (in nm) 502
Emission: (in nm) 521
Molecular Formula: C21H15ClN2O5
CAS Number: [N/A]
Unit Price (USD):
50 mg $140.03
Bulk Price/unit (when you buy 5 or more): $112.02
Description:
Stable, long wavelength, water soluble fluorophore for use in DNA-labeling, cell staining and protein labeling applications.
Note:
Ex = 492nm
Application:
Despite having a rhodamine nucleus, it has excitation and emission wavelengths similar to fluorescein (green color fluorescence), making it useful in detection systems employing fluorescein filter sets. For more information about this fluorophore, see the references below.
References:
"Optimization of spectroscopic and electrophoretic properties of energy transfer primers." Hung, S.C., Mathies, R.A., Glazer, A.N., Anal. Biochem. 252(1997)78-88."Characterization of visible dyes for four-decay fluorescence detection in DNA sequencing." Nunnally, B.K., He, H., Li, L.C., Tucker, S.A., McGown, L.B., Anal. Chem. 69(1997)2392-2397."Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution." Schwille, P., Meyer-Almes, F.J., Rigler, R., Biophys. J. 72(1997)1878-1886."Double-labeled donor probe can enhance the signal of fluorescence resonance energy transfer (FRET) in detection of nucleic acid hybridization." Okamura, Y., Kondo, S., Sase, I., Suga, T., Mise, K., Furusawa, I., Kawakami, S., Watanabe, Y., Nucleic Acids Res.28(2000)E107."DNA sequencing by capillary electrophoresis with four-decay fluorescence detection." He, H., McGown, L.B., Anal. Chem.72(2000)5865-5873."Effects of gel material on fluorescence lifetime detection of dyes and dye-labeled DNA primers in capillary electrophoresis." Li, L., McGown, L.B., J. Chromatogr. A 841(1999)95-103.
Keywords:
5(6)-Carboxyrhodamine 110, HCl salt, Carboxyrhodamine, 110, Marker Gene
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