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Product M0704
6-p-Toluidino-2-Naphthalenesulfonic acid (TNS)

6-p-Toluidino-2-Naphthalenesulfonic acid (TNS)
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Molecular Weight: 313.37
Storage: C,D,L
Soluble: DMSO, DMF, H2O (pH six+)
Absorption: (in nm) 318
Emission: (in nm) 443
Extinction (103): 26
Molecular Formula: C17H15NO3S
CAS Number: [7724-15-4]
Unit Price (USD):
500 mg $38.17
Bulk Price/unit (when you buy 5 or more): $30.54
Description: Fluorescent probe for conformation and quantitation studies of proteins.TNS is practically nonfluorescent in water, becoming fluorescent when bound to membranes (q.y. ~0.27) or proteins (q.y. ~0.72). Protein folding, conformational changes or other processes that modify the exposure of the probe to water can be monitored. The fluorescence is also enhanced by cyclodextrins, permitting a sensitive method for separating and analyzing cyclodextrins with capillary electrophoresis.
Note: Ex=318nm
References:
- "Fluorescent Probes for Conformational States of Proteins. I. Mechanism of Fluorescence of 2-p-Toluidinylnaphthalene-6-sulfonate, a Hydrophobic Probe" McClure, W.O., Edelman, G.M. Biochemistry 5(6) (1966) 1908-1919.
- "Determination of depolymerization kinetics of amylose, amylopectin, and soluble starch by aspergillus oryzae alpha-amylase using a fluorimetric 2-p-toluidinylnaphthalene-6-sulfonate/flow-injection analysis system." Batlle N, Carbonell JV, Sendra JM. Biotechnol Bioeng 70 (2000) 544-552.
- "Kinetics and Equilibrium Binding of the Dyes TNS and RH 421 to Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase (RUBISCO)." Frank J, et al. J. Chem. Soc. Faraday Trans. 93 (1997) 2379.
- "Use of 6-p-Toluidino-2-Naphthalenesulfonic Acid to Quantitate Lipids After Thin-Layer Chromatography." Jones M, Keenan RW, Horowitz P. J. Chromatogr. 237 (1982) 522.
- "pH-Induced conformational changes in Clostridium difficile toxin B." QaDan M, Spyres LM, Ballard JD. Infect. Immun. 68 (2000) 2470-2474.
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