Molecular Weight: 313.37

Storage: C, D, L

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO, DMF, H₂O (pH six+)

Absorption: (in nm) 318

Emission: (in nm) 443

Extinction (10³): 26

Molecular Formula: C₁₇H₁₅NO₃S

CAS Number: [7724-15-4]

Unit Price (USD): 500 mg $40.08

Bulk Price/unit (when you buy 5 or more): $32.07

Description:
Fluorescent probe for conformation and quantitation studies of proteins. Is practically nonfluorescent in water, becoming fluorescent when bound to membranes (q.y. ~0.27) or proteins (q.y. ~0.72).

Note:
Ex=318nm

Application:
Protein folding, conformational changes or other processes that modify the exposure of the probe to water can be monitored. The fluorescence is also enhanced by cyclodextrins, permitting a sensitive method for separating and analyzing cyclodextrins with capillary electrophoresis.

References:

• "Fluorescent Probes for Conformational States of Proteins. I. Mechanism of Fluorescence of 2-p-Toluidinylnaphthalene-6-sulfonate, a Hydrophobic Probe" McClure, W.O., Edelman, G.M. Biochemistry 5(6) (1966) 1908-1919.
• "Determination of depolymerization kinetics of amylose, amylopectin, and soluble starch by aspergillus oryzae alpha-amylase using a fluorimetric 2-p-toluidinylnaphthalene-6-sulfonate/flow-injection analysis system." Batlle N, Carbonell JV, Sendra JM. Biotechnol Bioeng 70 (2000) 544-552.
• "Kinetics and Equilibrium Binding of the Dyes TNS and RH 421 to Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase (RUBISCO)." Frank J, et al. J. Chem. Soc. Faraday Trans. 93 (1997) 2379.
• "Use of 6-p-Toluidino-2-Naphthalenesulfonic Acid to Quantitate Lipids After Thin-Layer Chromatography." Jones M, Keenan RW, Horowitz P. J. Chromatogr. 237 (1982) 522.
• "pH-Induced conformational changes in Clostridium difficile toxin B." QaDan M, Spyres LM, Ballard JD. Infect. Immun. 68 (2000) 2470-2474.