Molecular Weight: 423.46
Storage: F*, D, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO, DMF, water
Emission: (in nm) 470
Molecular Formula: C26H21N3O3
CAS Number: [55779-48-1]
Unit Price (USD):
50 μl* $44.21
Bulk Price/unit (when you buy 5 or more): $35.36
Description:
The native form of the luminescent enzyme substrate for apoaequorin, gaussia and renilla luciferases. This substrate is also a component of
our convenient assay kit, MarkerGene™ Gaussia Luciferase Cellular Assay Kit,
Product M1193, for quickly measuring the luciferase activity in mammalian cell culture.
Note:
*2.5mM solution in acidified MeOH. Store at -80oC for long periods.
Application:
This system has been used as a
very sensitive indicator of intracellular calcium ion concentrations, superoxide anion and peroxynitrite in cells or tissues. The coelenterazine/luciferase systems
from Renilla, Gaussia, Aequorea and Watesenia luciferases are widely used as marker genes in a variety of cell types.
The substrate, coelenterazine is common to many of the marine bioluminescent reactions, where it is either
utilized as a simple substrate, being catalytically turned over in the bioluminescent reaction,
or in others, such as in the photoprotein systems of Mneiopsis, it is incorporated as part of the photoprotein.
References:
McCapra and Beheshti in "Bioluminescence and Chemiluminescence: Instruments and Applications", 1985, Ed. K. Van Dyke, CRC Press, Boca Raton, FL. pgs. 9-42. Schuster, G.B., Schmidt, S.P. (1982) “Chemiluminescence of Organic Compounds” Adv. Phys. Org. Chem. 18: 187. Bakhos A. Tannous, Dong-Eog Kim, Juliet L. Fernandez, Ralph Weissleder and Xandra O. Breakefield (2005) “Codon-Optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and in Vivo” Molecular Therapy 11(3): 435-443.Verhaegent M, Christopoulos TK. (2002) “Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization.” Anal. Chem. 74(17): 4378-85.Kervinen M., Patsi J., Finel M., Hassinen I.E. (2004) "Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes." Anal. Biochem. 324: 45-51.Zhao H., Doyle T.C., Wong R.J., Cao Y., Stevenson D.K., Piwnica-Worms D., Contag C.H. (2004) "Characterization of coelenterazine analogs for measurements of Renilla luciferase activity in live cells and living animals." Mol. Imaging 3: 43-54.
Keywords:
Coelenterazine, apoaequorin, gaussia, renilla, luciferase, Marker Gene
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