Molecular Weight: 341.38

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO, H₂O

Molecular Formula: C₁₄H₁₉N₃O₅S

CAS Number: [35013-72-0]

Unit Price (USD): 100 mg $57.90

Bulk Price/unit (when you buy 5 or more): $46.32

Description:
Activated biotin derivative which can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions.

Application:
Biotin is a commonly used hapten that can be detected by using its strong affinity for the proteins avidin (from egg white) or streptavidin. Many avidin or streptavidin conjugates are available that have been fluorescently labeled or are conjugated to an antibody or other detection reagent. This biotin labeling compound has been used in many such detection schemes for peptides, proteins, organelle components, monoclonal antibody analyses, oligonucleotide and microbead labelings.

References:

• "Biotin-fluorophore conjugates with poly(ethylene glycol) spacers retain intense fluorescence after binding to avidin and streptavidin." Gruber, H.J., Marek, M., Schindler, H., Kaiser, K., Bioconjugate Chem. 8: 552-559 (1997).
• "Basic studies on heterobifunctional biotin-PEG conjugates with a 3-(4-pyridyldithio)propionyl marker on the second terminus." Kaiser K., Marek, M., Haselgrübler, T. Schindler, H., Gruber, H.J., Bioconjugate Chem. 8: 545-551. (1997).
• "Cyclic-AMP modulates downstream events in CD40-mediated signal transduction, but inhibition of protein kinase A has no direct effect on CD40 signaling." M.D. Goldstein, A. Cochrane and T.H. Watts, J. Immunol.159: 5871 (1997).
• "Coupling of monoclonal antibodies with biotin." Haugland, R.P., You, W.W., Methods Mol. Biol. 45: 223-233 (1995).