Molecular Weight: 668.4

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: H₂O, DMSO

Absorption: (in nm) 482

Emission: (in nm) 636

Extinction (10³): 5.4

Molecular Formula: C₂₇H₃₄N₄I₂

CAS Number: [25535-16-4]

Unit Price (USD): 150 μL $52.13

Bulk Price/unit (when you buy 5 or more): $41.70

Description:
Excellent counterstain for DNA and chromatin, providing a good contrast when also staining with green-fluorescent antibodies, or substrates.

Note:
Upon binding of propidium iodide to nucleic acids, the maximum excitation and emission wavelengths become EX 536 nm and EM 617 nm, respectively.

Application:
A unit contains 150 μL of a 2 mM solution in 1:4 DMSO:H2O. Propidium iodide is the most common red-fluorescent nuclear stain and can also be excited using excitation filters appropriate for green-fluorescent dyes. Green-fluorescent signals appear yellow when overlapping the red counterstaining. See also our M0795 for information about the use of propidium iodide in viability assay systems. Propidium iodide is membrane impermeant, and thus does not stain live cells. It also exhibits low background fluorescence from RNA samples in gels and in solution.

References:

• Qu, X., Chaires, J.B.,"Hydration changes for DNA intercalation reactions." (2001) J. Amer. Chem. Soc. 123: 1-7.
• Foglieni, C., Meoni, C., Davalli, A.M.,"Fluorescent dyes for cell viability: an application on prefixed conditions."(2001) Histochem. Cell Biol. 115:223-229.
• Smolewski, P., Grabarek, J., Kamentsky, L.A., Darzynkiewicz, Z.,"Bivariate analysis of cellular DNA versus RNA content by laser scanning cytometry using the product of signal subtraction (differential fluorescence) as a separate parameter." (2001) Cytometry 45:73-78.
• Comas-Riu, J., Vives-Rego, J.,"Cytometric monitoring of growth, sporogenesis and spore cell sorting in Paenibacillus polymyxa (formerly Bacillus polymyxa)." (2002) J. Appl. Microbiol. 92:475-81.