Molecular Weight: 255.23
Storage: C, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Absorption: (in nm) 500
Emission: (in nm) 593
Molecular Formula: C14H9NO4
CAS Number: [1152-14-3]
Unit Price (USD):
25 mg $66.29
Bulk Price/unit (when you buy 5 or more): $53.03
Description:
Common long-wavelength esterase substrate, useful in cell viability assays, or for high throughput screening of esterase activity in a live cell format.
Application:
Resorufin acetate is also a substrate for sheep liver cytosolic aldehyde dehydrogenase (CAD) as well as for assay of chymotrypsin activity, where the absorbance of the product resorufin M0202 is several times more intense than that formed by the widely used p-nitrophenyl acetate.
References:
Kitson, TM, Kitson KE, (1997) "Studies of the esterase activity of cytosolic aldehyde dehydrogenase with resorufin acetate as substrate." Biochem. J. 322: 701-708.
Kazlauskas, RJ (2006) "Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changesin Enzyme Assays",Jean-Louis Reymond, ed.,Wiley-VCH Verlag GmbH & Co. KGaA.
Bottcher D, Bornscheuer T, (2006) "High-throughput screening of activity and enantioselectivity of esterases." Nature Protocols 1: 2340-2343.
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