Molecular Weight: 487.3

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO, absolute EtOH

Molecular Formula: C₂₄H₁₆O₇Cl₂

CAS Number: [4091-99-0]

Unit Price (USD): 25 mg $46.30

Bulk Price/unit (when you buy 5 or more): $37.04

Description:
Reduced derivative of 2',7'-dichlorofluorescein is a cell permeable reagent for use in detection of peroxidase activity (oxidative metabolism, intracellular regulation of reactive oxygen species or oxidative burst) inside living cells.

Note:
Prepare solution in ethanol or DMSO and then add to media.

Application:
Upon enzyme activity, the highly fluorescent dye 2',7'-dichlorofluorescein Product M1278 is produced, with EX: 495nm and EM: 529 nm. See also Product M0545.

References:

• Kalivendi S.V., Kotamraju S., Cunningham S., Shang T., Hillard C.J., Kalyanaraman B. (2003) "1-Methyl-4-phenylpyridinium (MPP+)-induced apoptosis and mitochondrial oxidant generation: role of transferrin-receptor-dependent iron and hydrogen peroxide." Biochem J 371:151-64.
• Tampo Y., Kotamraju S., Chitambar C.R., Kalivendi S.V., Keszler A., Joseph J., Kalyanaraman B. (2003) "Oxidative stress-induced iron signaling is responsible for peroxide-dependent oxidation of dichlorodihydrofluorescein in endothelial cells: role of transferrin receptor-dependent iron uptake in apoptosis." Circ. Res. 92:56-63.
• He Y.Y., Hader D.(2002) "Reactive oxygen species and UV-B: effect on cyanobacteria." Photochem Photobiol Sci 1 729-36.
• Burkitt M.J., Wardman P. (2001) "Cytochrome C is a potent catalyst of dichlorofluorescin oxidation: implications for the role of reactive oxygen species in apoptosis." Biochem. Biophys. Res. Commun. 282 329-333
• Hail N. Jr, Lotan R. (2000) "Mitochondrial permeability transition is a central coordinating event in N-(4-hydroxyphenyl)retinamide-induced apoptosis." Cancer Epidemiol. Biomarkers Prev. 9 1293-1301.
• Salata R.A., Sullivan J.A., Mandell G.L.(1983) "Visualization of hydrogen peroxide in living polymorphonuclear neutrophils utilizing leucodiacetyl 2',7'-dichlorofluorescin: photomicrographic and microphotometric studies." Trans. Assoc. Am. Physicians 96 375-383.
• Keston AS, Brandt R.(1965) "The fluorometric analysis of ultramicro quantities of hydrogen peroxide." Anal. Biochem. 11: 1-5