Molecular Weight: 487.3

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO, absolute EtOH

Molecular Formula: C₂₄H₁₆O₇Cl₂

CAS Number: [4091-99-0]

Unit Price (USD): 25 mg $48.62

Bulk Price/unit (when you buy 5 or more): $38.89

Description:
Reduced derivative of 2',7'-dichlorofluorescein is a cell permeable reagent for use in detection of peroxidase activity (oxidative metabolism, intracellular regulation of reactive oxygen species or oxidative burst) inside living cells.

Note:
Prepare solution in ethanol or DMSO and then add to media.

Application:
Upon enzyme activity, the highly fluorescent dye 2',7'-dichlorofluorescein Product M1278 is produced, with EX: 495nm and EM: 529 nm. See also Product M0545.

References:

• Yu J, Xu Y, Khaoustov V, Yoffe B. (2010) "Identification of components of grape powder with anti-apoptotic effects."Toxicol Ind Health: published 10 August DOI: 10.1177/0748233710380220.
• Kalivendi SV, Kotamraju S, Cunningham S, Shang T, Hillard CJ, Kalyanaraman B. (2003) "1-Methyl-4-phenylpyridinium (MPP+)-induced apoptosis and mitochondrial oxidant generation: role of transferrin-receptor-dependent iron and hydrogen peroxide." Biochem J 371:151-64.
• Tampo Y, Kotamraju S, Chitambar CR, Kalivendi SV, Keszler A, Joseph J, Kalyanaraman B. (2003) "Oxidative stress-induced iron signaling is responsible for peroxide-dependent oxidation of dichlorodihydrofluorescein in endothelial cells: role of transferrin receptor-dependent iron uptake in apoptosis." Circ Res 92:56-63.
• He YY, Hader D.(2002) "Reactive oxygen species and UV-B: effect on cyanobacteria." Photochem Photobiol Sci 1: 729-36.
• Burkitt MJ, Wardman P. (2001) "Cytochrome C is a potent catalyst of dichlorofluorescin oxidation: implications for the role of reactive oxygen species in apoptosis." Biochem Biophys Res Commun 282: 329-333.
• Hail N Jr, Lotan R. (2000) "Mitochondrial permeability transition is a central coordinating event in N-(4-hydroxyphenyl)retinamide-induced apoptosis." Cancer Epidemiol Biomarkers Prev 9: 1293-1301.
• Salata RA, Sullivan JA, Mandell GL.(1983) "Visualization of hydrogen peroxide in living polymorphonuclear neutrophils utilizing leucodiacetyl 2',7'-dichlorofluorescin: photomicrographic and microphotometric studies." Trans Assoc Am Physicians 96: 375-383.
• Keston AS, Brandt R.(1965) "The fluorometric analysis of ultramicro quantities of hydrogen peroxide." Anal Biochem 11: 1-5.