Molecular Formula: N/A
CAS Number: [N/A]
Unit Price (USD):
1 kit $413.70
Bulk Price/unit (when you buy 5 or more): $413.70
Description:
Magic Red™ products are substrate-based assays designed to detect protease activity within whole living cells, using a fluorescence microscope.
Note:
| Product number | Magic Red™ Reagent | Target Caspase | Product Name | # Tests | Price | | M0837 | MR-(DEVD)2 | Caspases 3 and 7 | Magic Red™-(DEVD)2 Caspases 3 and 7 Assay Kit | 25 | $159.00 | | M0838 | MR-(DEVD)2 | Caspases 3 and 7 | Magic Red™-(DEVD)2 Caspases 3 and 7 Assay Kit | 100 | $394.00 |
Application:
Magic Red™ assays are based on a cresyl violet leaving group that fluoresces once the enzyme specific peptides are cleaved. No wash steps are required, making these kits very user friendly. In these kits, Magic Red™ is covalently attached to a specific peptide (DEVD) that when cleaved, causes the Magic Red™ to fluoresce.
References:
- Arends, M.J., Wylie, A.H., (1991) "Apoptosis: Mechanisms and roles in pathology." Int. Rev. Exp. Pathol. 32:223-254.
- Slee, E.A., Adrian, C., Martin, S.J., (1999) "Serial Kollers: Ordering Caspase Activation events in Apoptosis", Cell Death and Differ. 6: 1067-1074
- Salvesen, G.S., Dixit, V.M., (1997) "Caspases: intracellular signaling by proteoplysis", Cell 91:443-446.
- Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M.Labelle, E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A Thornberry and J. W. Becker. 1996. The three- dimensional structure of apopain/ CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3( 7): 619- 625.
- Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060- 1066.
- Thornberry, N. A., T. A. Rano, E. P. Peterson, D. M. Rasper, T. Timkey, M. Garcia- Calvo, V. M. Houtszager, P. A. Nordstrom, S. Roy, J. P.Vaillancourt, K. T. Chapman and D. W. Nicholson. 1997. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J. Biol. Chem. 272( 29): 17907- 17911.
- Ekert, P. G., J. Silke and D. L. Vaux. 1999. Caspase inhibitors. Cell Death and Differ. 6: 1081- 1086.
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