Unit Price (USD):
1 kit $240.24
Bulk Price/unit (when you buy 5 or more): $192.19
Description:
Allows for quantitative measure of β-galactosidase enzyme activity in mammalian, yeast, and bacterial cells through use of a chemiluminescent substrate.
Application:
One of the most common reporter genes used in molecular biology applications is the E. coli lacZ gene that codes for an active subunit of β-galactosidase in vivo. Since this enzyme is generally absent in normal mammalian, yeast, some bacterial and even plant cells, it can be detected at very low levels, and due to its wide substrate specificity, monitoring lacZ expression (and therefore co-expressed genes or promoter efficiency) has become routine to the point of detection of as few as 5 copies of β-galactosidase. Although chromogenic assays of β-galactosidase activity (i.e. X-Gal) are useful, the recent application of chemiluminescent 1,2 Dioxetane substrates, which emit visible light upon enzyme catalysis, provide rapid results with very low background and high intensity signal. An enhancing solution is also provided with this kit to increase light production efficiency in solution-based assays by drawing water away from the signal production site. The Chemiluminescent lacZ β-Galactosidase Detection Kit provides all the necessary reagents, buffers, substrate, and protocols for sensitive and quantitative lacZ β-galactosidase activity assay. Please contact our technical services department for further information.
References:
Bukh AS, Roslev P (2010) "Characterization and validation of a chemiluminescent assay based on 1,2-dioxetanes for rapid detection of viable Escherichia coli" Appl. Microbiol. Biotechnol. (2010) 86:1947–1957
van Poucke SO, Nelis HJ (1995) "Development of a Sensitive Chemiluminometric Assay for the Detection of b-Galactosidase in Permeabilized Coliform Bacteria and Comparison with Fluorometry and Colorimetry" Appl. Environ. Microbiol. 1995: 4505-4509.
Chemiluminescent 1,2-dioxetanes. Brij P. Giri, PCT/US99/20590."Novel stabilized formulations for chemiluminescent assays." Brij P Giri, PCT/US01/02779."Single molecule detection of Alkaline Phosphatase enzyme using enhanced chemiluminescent from 1,2-dioxetanes and water-soluble, water-insoluble or partially water-soluble polymers." Brij P Giri, US Patent Application, 2002/0013250. "Deuterium-based chemiluminescent 1,2-dioxetanes." Brij P Giri, Dinesh J Dagli, PCT application pending. "Polymeric ammonium and or phosphonium salts having added p-electrons and higher molecular weight and enhancers for chemiluminescent systems."Brij P. Giri, US patent pending.
|
