CAS Number: [N/A]
Unit Price (USD):
20 ug $127.05
Bulk Price/unit (when you buy 5 or more): $127.05
Description:
Common eukaryotic expression vector, pSV40β, expresses the full-length β-galactosidase gene under the control of simian virus 40 (SV40) early promoter. Very useful for transfection of mammalian cells in culture and for use in other species.
Note:
Full Length Sequence Data
Restriction
Sites
Application:
The β-galactosidase enzyme expression is enhanced by elements including: SD/SA-RNA splice donor and acceptor sequence, and SV40 late polyadenylylation signal. pSV40β expression vector also contains β-lactamase, which acts as a selection marker (100 ug/ml ampicillin resistance) in E. coli host. See also our pCMVβ vector system, M0951.
References:
Hall, C.V. et al., 1983. "Expression and Regulation of Escherichia coli lacZ Gene Fusions in Mammalian Cells." J. Mol. Appl. Gen. 2:101.
Herbomel, P. et al., 1984. "Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma." Cell 39:653.
Nolan, G.P., et al., 1988. "Fluorescence-activated Cell Analysis and Sorting of Viable Mammalian Cells Based on b-D-galactosidase Activity after Transduction of Escherichia coli lacZ." Proc. Natl. Acad. Sci (USA). 85:2603.
Nikcevic, G. et al., 2003. "Improved transfection efficiency of cultured human cells." Cell Biol. Int. 27:735.
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