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Description:
Provides reagents and methods to simply and quickly label antibodies or other proteins with the amine-reactive dye 5(6)-carboxytetramethylrhodamine.
Application:
Direct labeling of a primary antibody eliminates the need to use a secondary Ab, providing lower background and higher sensitivity. The TAMRA dye provided in this kit forms the desired dye-protein conjugates by reacting with the primary amines of proteins, giving absorption and emission maxima of 555nm and 580nm, respectively. The kit contains enough reagents for 5 labelings, including buffers, solvents, and a detailed protocol for use. Please contact our technical services department for further information.
References:
View References ▼
- Perrier AL, Massoulie J, Krejci E., (2002) "PRiMA: the membrane anchor of acetylcholinesterase in the brain." Neuron. 33: 275-285.
- Peng HB, Xie H, Rossi SG, Rotundo RL., (1999) "Acetylcholinesterase clustering at the neuromuscular junction involves perlecan and dystroglycan." J Cell Biol. 145: 911-921.
- Vamosi G, Gohlke C, Clegg RM., (1996) "Fluorescence characteristics of 5-carboxytetramethylrhodamine linked covalently to the 5 end of oligonucleotides: multiple conformers of single-stranded and double-stranded dye-DNA complexes." Biophys J. 71(2): 972-74.
- Kwon G, Remmers AE, Datta S, Neubig RR. (1993) "Synthesis and characterization of fluorescently labeled bovine brain G protein subunits." Biochemistry. 32(9): 2401-8.
- Brinkley, M., (1992) "A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents."Bioconjug Chem. 3(1): 2-13.
- Haugland, R.P., (1995) "Coupling of monoclonal antibodies with fluorophores." Methods Mol Biol. 45: 205-221.
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Unit Price (USD):
1 kit $211.02
Bulk Price/unit
(5 or more): $168.82
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