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M1025 - 4-Hydrazino-7-nitrobenzofurazane
(NBD-hydrazine)


Molecular Weight: 195.14

Storage: C, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Emission: (in nm) 522

Molecular Formula: C6H5N5O3

CAS Number: [90421-78-6]

Alternative Name: NBD-hydrazine

Unit Price (USD): 25 mg $53.48

Bulk Price/unit (when you buy 5 or more): $42.78

Description:
Fluorescent derivatization reagent useful for aliphatic aldehyde detection including reducing carbohydrates.

Note:
Ex= 445nm. EX/EM after derivatization with formaldehyde

References:

  • "Assay for synthesis activity of hydrolases in nonaqueous media using enzymic transesterification reaction releasing a carbonyl compd." Bornscheuer UT, Kanarzycka M, Hills G. Eur. Pat. Appl. EP 1354960 (2003).
  • "Solid reagent for sampling and derivatization of carbonyl compounds." Appelblad P, Irgum K, Ponten E. PCT Int. Appl. WO 2002094769 (2002).
  • "Semi-empirical PM3 calculation reveals the relationship between the fluorescence characteristics of 4,7-disubstituted benzofurazan compounds, the LUMO energy and the dipole moment directed from the 4- to the 7-position." Uchiyama S, Santa T, Imai K. J Chem. Soc., Perkin Transactions 2: Physical Organic Chemistry 3: 569-576 (1999).
  • "Effects of the substituent groups at the 4- and 7-positions on the fluorescence characteristics of benzofurazan compounds." Uchiyama S, Santa T, Fukushima T, Homma H, Imai K. J Chem. Soc., Perkin Transactions 2: Physical Organic Chemistry 10: 2165-2174 (1998).
  • "High-performance liquid chromatography of aliphatic aldehydes by means of post-column extraction with fluorometric detection." Koizumi H, Suzuki Y. J of Chromatography 457: 299-307 (1988).
  • "Fluorogenic labeling of carbonyl compounds with 7-hydrazino-4-nitro-2,1,3-benzoxadiazole (NBD-H)." Guebitz G, Wintersteiger R, Frei RW. J of Liquid Chromatography 7(4): 839-54 (1984).


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