Molecular Weight: 560.4

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO

Absorption: (in nm) 488

Emission: (in nm) 512

Molecular Formula: C₂₀H₂₆N₄O₁₁P₂

CAS Number: [217305-49-2]

Unit Price (USD): 5 mg $218.82

Bulk Price/unit (when you buy 5 or more): $175.06

Description:
Most widely used ultrasensitive fluorogenic substrate for alkaline phosphatases.

Note:
Abs/Em of fluorescein upon alkaline phosphatase activity.

References:

• "Measuring the specific activity of the CD45 protein tyrosine phosphatase." Rider DA, Young SP. J Immunol Methods 277: 127-134 (2003).
• "Development of fluorescence-based selective assays for serine/threonine and tyrosine phosphatases." Pastula C, Johnson I, Beechem JM, Patton WF. Comb Chem High Throughput Screen 6: 341-346 (2003).
• "Development of a method for evaluating protein tyrosine phosphatase CD45 inhibitors using jurkat cell membrane." Waddleton D, Ramachandran C, Wang Q. Anal Biochem 285: 58-63 (2000).
• "3,6-Fluorescein diphosphate: a sensitive fluorogenic and chromogenic substrate for protein tyrosine phosphatases." Huang Z, Wang Q, Ly HD, Gorvindarajan A, Scheigetz J, Zamboni R, Desmarais S, Ramachandran C. J Biomol Screen 4(6): 327-334 (1999).
• "Fluorescein monophosphates as fluorogenic substrates for protein tyrosine phosphatases." Wang Q, Scheigetz J, Gilbert M, Snider J, Ramachandran C. Biochim Biophys Acta 1431: 14-23 (1999).
• "Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from fluorescein (3,6-dihydroxyfluoran) and its monomethyl ether." Rotman B, Zderic JA, Edelstein M. Proc Natl Acad Sci USA 50: 1 (1963).