Molecular Weight: 421.43

Storage: F, D, L

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMF,H₂O

Absorption: (in nm) 492

Emission: (in nm) 516

Extinction (10³): 85

Molecular Formula: C₂₁H₁₅N₃O₅S

CAS Number: [76863-28-0]

Unit Price (USD): 50 mg $72.98

Bulk Price/unit (when you buy 5 or more): $58.38

Description:
Cell impermeant fluorescent probe useful for determining protein and peptide topology on the cell surface.

Note:
Abs/Em in 0.1M Tris, pH=9

References:

• "A new strategy for site-specific protein modification: analysis of a Tat peptide-TAR RNA interaction." Tamilarasu N, Zhang J, Hwang S, Rana TM. Bioconjug Chem 12: 135-138 (2001).
• "Improved Method for Detecting Fluorescent-Labeled Glycoprotein on Sodium Dodecyl Sulfate-Polyacrylamide Gel." Gao X, Liu W-Y, Ruan K. Bioorg Chem 25: 163 (1997).
• "A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel." Wu TP, Ruan KC, Liu WY. Nucleic Acids Res 24: 3472-3 (1996).
• "Electrostatic potential of macromolecules measured by pKa shift of a fluorophore. 1. The 3 terminus of 16S RNA." Friedrich K, Woolley P. Eur J Biochem 173: 227-231 (1988).
• "A simple method for labelling the carbohydrate moieties of antibodies with fluorochromes." Duijndam WA, Wiegant J, Van Duijn P, Haaijman JJ. J Immunol Methods 109: 289-290 (1988).
• "Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels." Ahn B, Rhee SG, Stadtman ER. Anal Biochem 161: 245-257 (1987).
• "Direct covalent linkage of fluorescent probes to the plant protoplast surface." Furtula V, Walko RM, Nothnagel EA. Protoplasma 139: 117 (1987).
• "Distances between 3 ends of ribosomal ribonucleic acids reassembled into Escherichia coli ribosomes." Odom OW Jr, Robbins DJ, Lynch J, Dottavio-Martin D, Kramer G, Hardesty B. Biochemistry 9: 5947-5954 (1980).